shrna-designer-v1
Enter (Human, Mouse, or Rat) Accession Number: | |||
Enter sequence: | |||
Minimum GC% | Maximum GC% | ||
| |||
Additional Notes: |
*Biosettia grades each designed oligo based on the probability that it will knock down your target RNA by at least 70%. |
Grades are based on a combination of a modified Ui-Tei scoring method as well as free energy values of both terminal and overall regions of each oligo. |
shRNA Converter | |||||
Convert Your Own siRNA into shRNA | |||||
Paste your 19-21nt sequence here to construct a compatible oligo for use with Biosettia's vectors: |
References: |
1. Taxman, Debra J., et al., Criteria for effective design, construction, and gene knockdown by shRNA vectors. BMC Biotechnology, 2006. 6:7. |
2. Matveeva, O.V., et al., Optimization of duplex stability and terminal asymmetry for shRNA design. PLoS One, 2010, Apr 20;5(4):e10180. |