Biosettia, a better solution in life science

Examples of Knockdown by pLV-RNAi Vectors

 

Examples of Gene Silencing with Biosettia’s pLV-RNAi Vectors:



The shRNAs targeting genes of interest were cloned into pLV-RNAi vector system to generate lenti-shRNA viral stocks. The lentiviruses were then used to transduce the cell lines of choice. The cell lysates were collected at 2 days post-transduction, separated on SDS-PAGE gels, and subjected to Western blot analyses. The negative control (neg. ctrl.) is an shRNA inhibiting LacZ gene expression.

Examples of Gene Silencing with Inducible pLV-RNAi Vectors:



The shRNAs targeting genes of interest were cloned into the inducible pLV-RNAi vectors to generate lenti-shRNA viral stocks. The lentiviruses were then used to transduce the cell lines of choice. Doxycycline (Dox) was added to the transduced cells at 4 days post-transduction. The cell lysates were collected at 3 days after Dox induction, separated on SDS-PAGE gels, and subjected to Western blot analyses. The negative control (neg. ctrl.) is an shRNA inhibiting LacZ gene expression.