pLV-RNAi Protocols – Confirmation of knockdown

Confirmation of knockdown and RNAi analysis.

Confirmation of knockdown of the specific target gene is an essential step to ensure the success of an RNAi experiment. The approaches to validating effective shRNAs include the demonstration that the expression level of the target mRNA and/or gene product is substantially reduced while a negative control shRNA shows no effect. Optionally, a reporter assay can be applied to screen potent shRNAs before packaging pLV-RNAi recombinants into lentiviruses.

The flow chart below illustrates an overview of the options and strategies to analyze RNAi efficacy with the pLV-RNAi vector.