LOCUS       pRNAi-mU6-green         4259 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     rep_origin      3368..3956
                     /gene=pUC ori
     CDS             2337..3197
                     /gene=Amp(R)
     rep_origin      401..857
                     /gene=f1 ori
     promoter        864..1228
                     /gene=SV40 early promoter
     polyA_signal    1998..2128
                     /gene=SV40 early polyA
     promoter        16..330
                     /gene=mouse U6 promoter
     CDS             1259..1978
                     /gene=GFP
     insertion_seq   331..382
                     /gene=shRNA
                     
BASE COUNT     1105 a      1078 c      1052 g       982 t        42 others
ORIGIN
        1 cccgggacag aattcgatcc gacgccgcca tctctaggcc cgcgccggcc ccctcgcaca 
       61 gacttgtggg agaagctcgg ctactcccct gccccggtta atttgcatat aatatttcct 
      121 agtaactata gaggcttaat gtgcgataaa agacagataa tctgttcttt ttaatactag 
      181 ctacatttta catgataggc ttggatttct ataagagata caaatactaa attattattt 
      241 taaaaaacag cacaaaagga aactcaccct aactgtaaag taattgtgtg ttttgagact 
      301 ataaatatcc cttggagaaa agcctaaaaa nnnnnnnnnn nnnnnnnnnn nttggatcca 
      361 annnnnnnnn nnnnnnnnnn nntttttttt aagcttcacc ttgtaagcgt taatattttg 
      421 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc 
      481 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt 
      541 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 
      601 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg 
      661 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga 
      721 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 
      781 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg 
      841 ctacagggcg cgtcaggctc gagttctgag gcggaaagaa ccagctgtgg aatgtgtgtc 
      901 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 
      961 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 
     1021 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 
     1081 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 
     1141 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 
     1201 ttggaggcct aggcttttgc aaaaagctcc cggtaatacg actcactata gggctagcat 
     1261 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg 
     1321 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg 
     1381 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct 
     1441 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca 
     1501 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt 
     1561 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt 
     1621 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa 
     1681 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg 
     1741 catcaaggtg aacttcgaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga 
     1801 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta 
     1861 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct 
     1921 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagc 
     1981 ggccgcaatc tagaccaaac ttgtttattg cagcttataa tggttacaaa taaagcaata 
     2041 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 
     2101 aactcatcaa tgtatcttat catgtctgtg atcaggtacc aaagggcctc gtgatacgcc 
     2161 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc 
     2221 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 
     2281 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 
     2341 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 
     2401 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 
     2461 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 
     2521 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 
     2581 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 
     2641 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 
     2701 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 
     2761 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 
     2821 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 
     2881 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 
     2941 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 
     3001 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 
     3061 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 
     3121 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 
     3181 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 
     3241 aacttcattt ttaattaaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 
     3301 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 
     3361 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 
     3421 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 
     3481 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc 
     3541 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 
     3601 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 
     3661 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 
     3721 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 
     3781 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 
     3841 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 
     3901 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 
     3961 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 
     4021 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 
     4081 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 
     4141 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg 
     4201 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctc 
//
