LOCUS       pRNAi-hU6-green         4208 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     rep_origin      3317..3905
                     /gene=pUC ori
     CDS             2286..3146
                     /gene=Amp(R)
     rep_origin      350..806
                     /gene=f1 ori
     promoter        16..279
                     /gene=human U6 promoter
     promoter        813..1177
                     /gene=SV40 early promoter
     polyA_signal    1947..2077
                     /gene=SV40 early polyA
     CDS             1208..1927
                     /gene=GFP
     insertion_seq   280..331
                     /gene=shRNA
                     
BASE COUNT     1095 a      1043 c      1048 g       980 t        42 others
ORIGIN
        1 cccgggacag aattcaaggt cgggcaggaa gagggcctat ttcccatgat tccttcatat 
       61 ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca 
      121 aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt 
      181 ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat 
      241 ttcttggctt tatatatctt gtggaaagga cgaaaaaaan nnnnnnnnnn nnnnnnnnnn 
      301 ttggatccaa nnnnnnnnnn nnnnnnnnnn ntttttttta agcttcacct tgtaagcgtt 
      361 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag 
      421 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 
      481 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 
      541 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 
      601 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 
      661 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 
      721 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 
      781 aatgcgccgc tacagggcgc gtcaggctcg agttctgagg cggaaagaac cagctgtgga 
      841 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 
      901 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 
      961 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 
     1021 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 
     1081 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 
     1141 gaggcttttt tggaggccta ggcttttgca aaaagctccc ggtaatacga ctcactatag 
     1201 ggctagcatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 
     1261 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 
     1321 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 
     1381 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca 
     1441 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 
     1501 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 
     1561 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 
     1621 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca 
     1681 gaagaacggc atcaaggtga acttcgagat ccgccacaac atcgaggacg gcagcgtgca 
     1741 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 
     1801 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca 
     1861 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 
     1921 caagtaagcg gccgcaatct agaccaaact tgtttattgc agcttataat ggttacaaat 
     1981 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 
     2041 gtttgtccaa actcatcaat gtatcttatc atgtctgtga tcaggtacca aagggcctcg 
     2101 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 
     2161 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 
     2221 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 
     2281 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 
     2341 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 
     2401 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 
     2461 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 
     2521 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 
     2581 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 
     2641 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 
     2701 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 
     2761 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 
     2821 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 
     2881 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 
     2941 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 
     3001 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 
     3061 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 
     3121 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 
     3181 ttgatttaaa acttcatttt taattaaaaa ggatctaggt gaagatcctt tttgataatc 
     3241 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 
     3301 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 
     3361 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 
     3421 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 
     3481 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 
     3541 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 
     3601 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 
     3661 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 
     3721 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 
     3781 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 
     3841 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 
     3901 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 
     3961 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 
     4021 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 
     4081 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 
     4141 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga 
     4201 gttagctc 
//
