LOCUS       pRNAi-H1-green          4167 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     rep_origin      3276..3864
                     /gene=pUC ori
     CDS             2245..3105
                     /gene=Amp(R)
     rep_origin      309..765
                     /gene=f1 ori
     promoter        772..1136
                     /gene=SV40 early promoter
     polyA_signal    1906..2036
                     /gene=SV40 early polyA
     promoter        10..238
                     /gene=H1 promoter
     CDS             1167..1886
                     /gene=GFP
     insertion_seq   239..290
                     /gene=shRNA
                     
BASE COUNT     1059 a      1063 c      1061 g       942 t        42 others
ORIGIN
        1 cccgggacag aattcgaacg ctgacgtcat caacccgctc caaggaatcg cgggcccagt 
       61 gtcactaggc gggaacaccc agcgcgcgtg cgccctggca ggaagatggc tgtgagggac 
      121 aggggagtgg cgccctgcaa tatttgcatg tcgctatgtg ttctgggaaa tcaccataaa 
      181 cgtgaaatgt ctttggattt gggaatctta taagttctgt atgagaccac tctaaaaann 
      241 nnnnnnnnnn nnnnnnnnnt tggatccaan nnnnnnnnnn nnnnnnnnnn ttttttttaa 
      301 gcttcacctt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 
      361 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 
      421 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 
      481 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 
      541 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 
      601 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 
      661 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 
      721 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggctcga gttctgaggc 
      781 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 
      841 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 
      901 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 
      961 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 
     1021 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 
     1081 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg 
     1141 gtaatacgac tcactatagg gctagcatgg tgagcaaggg cgaggagctg ttcaccgggg 
     1201 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg 
     1261 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg 
     1321 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct 
     1381 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag 
     1441 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 
     1501 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 
     1561 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct 
     1621 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcgagatc cgccacaaca 
     1681 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 
     1741 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc 
     1801 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 
     1861 tcggcatgga cgagctgtac aagtaagcgg ccgcaatcta gaccaaactt gtttattgca 
     1921 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 
     1981 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtgat 
     2041 caggtaccaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg 
     2101 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta 
     2161 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt 
     2221 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc 
     2281 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa 
     2341 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt 
     2401 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt 
     2461 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc 
     2521 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg 
     2581 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg 
     2641 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac 
     2701 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca 
     2761 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta 
     2821 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat 
     2881 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa 
     2941 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag 
     3001 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat 
     3061 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt 
     3121 tactcatata tactttagat tgatttaaaa cttcattttt aattaaaaag gatctaggtg 
     3181 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 
     3241 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 
     3301 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 
     3361 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 
     3421 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 
     3481 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 
     3541 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 
     3601 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 
     3661 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 
     3721 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 
     3781 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 
     3841 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 
     3901 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 
     3961 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc 
     4021 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt 
     4081 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag 
     4141 cgcaacgcaa ttaatgtgag ttagctc 
//
