LOCUS       pRNAi-mU6-bsd           3938 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     rep_origin      3047..3635
                     /gene=pUC ori
     CDS             2016..2876
                     /gene=Amp(R)
     rep_origin      401..857
                     /gene=f1 ori
     promoter        864..1228
                     /gene=SV40 early promoter
     polyA_signal    1677..1807
                     /gene=SV40 early polyA
     CDS             1259..1657
                     /gene=Bsd(R)
     promoter        16..330
                     /gene=mouse U6 promoter
     insertion_seq   331..382
                     /gene=shRNA
                     
BASE COUNT     1021 a       943 c       962 g       970 t        42 others
ORIGIN
        1 cccgggacag aattcgatcc gacgccgcca tctctaggcc cgcgccggcc ccctcgcaca 
       61 gacttgtggg agaagctcgg ctactcccct gccccggtta atttgcatat aatatttcct 
      121 agtaactata gaggcttaat gtgcgataaa agacagataa tctgttcttt ttaatactag 
      181 ctacatttta catgataggc ttggatttct ataagagata caaatactaa attattattt 
      241 taaaaaacag cacaaaagga aactcaccct aactgtaaag taattgtgtg ttttgagact 
      301 ataaatatcc cttggagaaa agcctaaaaa nnnnnnnnnn nnnnnnnnnn nttggatcca 
      361 annnnnnnnn nnnnnnnnnn nntttttttt aagcttcacc ttgtaagcgt taatattttg 
      421 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc 
      481 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt 
      541 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 
      601 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg 
      661 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga 
      721 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 
      781 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg 
      841 ctacagggcg cgtcaggctc gagttctgag gcggaaagaa ccagctgtgg aatgtgtgtc 
      901 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 
      961 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 
     1021 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 
     1081 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 
     1141 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 
     1201 ttggaggcct aggcttttgc aaaaagctcc cggtaatacg actcactata gggctagcat 
     1261 ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa 
     1321 cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg 
     1381 catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt 
     1441 gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa 
     1501 tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca 
     1561 tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg 
     1621 tgaattgctg ccctctggtt atgtgtggga gggctaagcg gccgcaatct agaccaaact 
     1681 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 
     1741 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 
     1801 atgtctgtga tcaggtacca aagggcctcg tgatacgcct atttttatag gttaatgtca 
     1861 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 
     1921 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 
     1981 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 
     2041 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 
     2101 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 
     2161 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 
     2221 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 
     2281 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 
     2341 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 
     2401 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 
     2461 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 
     2521 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 
     2581 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 
     2641 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 
     2701 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 
     2761 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 
     2821 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 
     2881 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taattaaaaa 
     2941 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 
     3001 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 
     3061 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 
     3121 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 
     3181 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 
     3241 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 
     3301 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 
     3361 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 
     3421 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 
     3481 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 
     3541 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 
     3601 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 
     3661 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 
     3721 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 
     3781 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc 
     3841 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 
     3901 cgggcagtga gcgcaacgca attaatgtga gttagctc 
//
