LOCUS       pRNAi-hU6-bsd           3887 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     rep_origin      2996..3584
                     /gene=pUC ori
     CDS             1965..2825
                     /gene=Amp(R)
     rep_origin      350..806
                     /gene=f1 ori
     promoter        16..279
                     /gene=human U6 promoter
     promoter        813..1177
                     /gene=SV40 early promoter
     polyA_signal    1626..1756
                     /gene=SV40 early polyA
     CDS             1208..1606
                     /gene=Bsd(R)
     insertion_seq   280..331
                     /gene=shRNA
                     
BASE COUNT     1011 a       908 c       958 g       968 t        42 others
ORIGIN
        1 cccgggacag aattcaaggt cgggcaggaa gagggcctat ttcccatgat tccttcatat 
       61 ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca 
      121 aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt 
      181 ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat 
      241 ttcttggctt tatatatctt gtggaaagga cgaaaaaaan nnnnnnnnnn nnnnnnnnnn 
      301 ttggatccaa nnnnnnnnnn nnnnnnnnnn ntttttttta agcttcacct tgtaagcgtt 
      361 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag 
      421 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 
      481 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 
      541 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 
      601 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 
      661 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 
      721 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 
      781 aatgcgccgc tacagggcgc gtcaggctcg agttctgagg cggaaagaac cagctgtgga 
      841 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 
      901 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 
      961 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 
     1021 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 
     1081 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 
     1141 gaggcttttt tggaggccta ggcttttgca aaaagctccc ggtaatacga ctcactatag 
     1201 ggctagcatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc 
     1261 tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag 
     1321 cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga 
     1381 actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc 
     1441 gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct 
     1501 cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt 
     1561 tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagcgg ccgcaatcta 
     1621 gaccaaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 
     1681 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 
     1741 tatcttatca tgtctgtgat caggtaccaa agggcctcgt gatacgccta tttttatagg 
     1801 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc 
     1861 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac 
     1921 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt 
     1981 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag 
     2041 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg 
     2101 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa 
     2161 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc 
     2221 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag 
     2281 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa 
     2341 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc 
     2401 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg 
     2461 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa 
     2521 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa 
     2581 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg 
     2641 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag 
     2701 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg 
     2761 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt 
     2821 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt 
     2881 aattaaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 
     2941 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 
     3001 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 
     3061 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 
     3121 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga 
     3181 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 
     3241 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 
     3301 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 
     3361 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 
     3421 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 
     3481 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 
     3541 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 
     3601 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 
     3661 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca 
     3721 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca 
     3781 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg 
     3841 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctc 
//
