LOCUS       pRNAi-H1-bsd            3846 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     rep_origin      2955..3543
                     /gene=pUC ori
     CDS             1924..2784
                     /gene=Amp(R)
     rep_origin      309..765
                     /gene=f1 ori
     promoter        772..1136
                     /gene=SV40 early promoter
     polyA_signal    1585..1715
                     /gene=SV40 early polyA
     promoter        10..238
                     /gene=H1 promoter
     CDS             1167..1565
                     /gene=Bsd(R)
     insertion_seq   239..290
                     /gene=shRNA
                     
BASE COUNT      975 a       928 c       971 g       930 t        42 others
ORIGIN
        1 cccgggacag aattcgaacg ctgacgtcat caacccgctc caaggaatcg cgggcccagt 
       61 gtcactaggc gggaacaccc agcgcgcgtg cgccctggca ggaagatggc tgtgagggac 
      121 aggggagtgg cgccctgcaa tatttgcatg tcgctatgtg ttctgggaaa tcaccataaa 
      181 cgtgaaatgt ctttggattt gggaatctta taagttctgt atgagaccac tctaaaaann 
      241 nnnnnnnnnn nnnnnnnnnt tggatccaan nnnnnnnnnn nnnnnnnnnn ttttttttaa 
      301 gcttcacctt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 
      361 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 
      421 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 
      481 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 
      541 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 
      601 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 
      661 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 
      721 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggctcga gttctgaggc 
      781 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 
      841 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 
      901 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 
      961 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 
     1021 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 
     1081 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg 
     1141 gtaatacgac tcactatagg gctagcatgg ccaagccttt gtctcaagaa gaatccaccc 
     1201 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg 
     1261 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta 
     1321 ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca 
     1381 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac 
     1441 ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg 
     1501 atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg 
     1561 gctaagcggc cgcaatctag accaaacttg tttattgcag cttataatgg ttacaaataa 
     1621 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 
     1681 ttgtccaaac tcatcaatgt atcttatcat gtctgtgatc aggtaccaaa gggcctcgtg 
     1741 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc 
     1801 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat 
     1861 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 
     1921 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt 
     1981 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt 
     2041 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc 
     2101 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta 
     2161 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac 
     2221 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa 
     2281 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg 
     2341 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc 
     2401 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg 
     2461 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta 
     2521 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg 
     2581 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg 
     2641 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc 
     2701 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt 
     2761 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt 
     2821 gatttaaaac ttcattttta attaaaaagg atctaggtga agatcctttt tgataatctc 
     2881 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 
     2941 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 
     3001 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 
     3061 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag 
     3121 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 
     3181 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 
     3241 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 
     3301 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 
     3361 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 
     3421 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 
     3481 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 
     3541 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 
     3601 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga 
     3661 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg 
     3721 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 
     3781 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt 
     3841 tagctc 
//
