LOCUS       pRNAi-mU6-neo           4334 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     rep_origin      3443..4031
                     /gene=pUC ori
     CDS             2412..3272
                     /gene=Amp(R)
     rep_origin      401..857
                     /gene=f1 ori
     promoter        864..1228
                     /gene=SV40 early promoter
     polyA_signal    2073..2203
                     /gene=SV40 early polyA
     promoter        16..330
                     /gene=mouse U6 promoter
     CDS             1259..2053
                     /gene=Neo(R)
     insertion_seq   331..382
                     /gene=shRNA
                     
BASE COUNT     1077 a      1063 c      1096 g      1056 t        42 others
ORIGIN
        1 cccgggacag aattcgatcc gacgccgcca tctctaggcc cgcgccggcc ccctcgcaca 
       61 gacttgtggg agaagctcgg ctactcccct gccccggtta atttgcatat aatatttcct 
      121 agtaactata gaggcttaat gtgcgataaa agacagataa tctgttcttt ttaatactag 
      181 ctacatttta catgataggc ttggatttct ataagagata caaatactaa attattattt 
      241 taaaaaacag cacaaaagga aactcaccct aactgtaaag taattgtgtg ttttgagact 
      301 ataaatatcc cttggagaaa agcctaaaaa nnnnnnnnnn nnnnnnnnnn nttggatcca 
      361 annnnnnnnn nnnnnnnnnn nntttttttt aagcttcacc ttgtaagcgt taatattttg 
      421 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc 
      481 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt 
      541 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 
      601 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg 
      661 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga 
      721 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 
      781 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg 
      841 ctacagggcg cgtcaggctc gagttctgag gcggaaagaa ccagctgtgg aatgtgtgtc 
      901 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 
      961 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 
     1021 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 
     1081 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 
     1141 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 
     1201 ttggaggcct aggcttttgc aaaaagctcc cggtaatacg actcactata gggctagcat 
     1261 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 
     1321 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 
     1381 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 
     1441 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 
     1501 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 
     1561 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 
     1621 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 
     1681 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 
     1741 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg 
     1801 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 
     1861 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 
     1921 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 
     1981 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 
     2041 cgagttcttc tgagcggccg caatctagac caaacttgtt tattgcagct tataatggtt 
     2101 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 
     2161 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtgatcag gtaccaaagg 
     2221 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt 
     2281 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 
     2341 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 
     2401 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 
     2461 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 
     2521 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 
     2581 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 
     2641 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 
     2701 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 
     2761 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 
     2821 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 
     2881 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 
     2941 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 
     3001 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 
     3061 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 
     3121 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 
     3181 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 
     3241 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 
     3301 tttagattga tttaaaactt catttttaat taaaaaggat ctaggtgaag atcctttttg 
     3361 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 
     3421 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 
     3481 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 
     3541 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt 
     3601 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 
     3661 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 
     3721 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 
     3781 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 
     3841 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 
     3901 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 
     3961 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 
     4021 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 
     4081 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 
     4141 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 
     4201 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 
     4261 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 
     4321 atgtgagtta gctc 
//
