LOCUS       pRNAi-hU6-neo           4283 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     rep_origin      3392..3980
                     /gene=pUC ori
     CDS             2361..3221
                     /gene=Amp(R)
     rep_origin      350..806
                     /gene=f1 ori
     promoter        16..279
                     /gene=human U6 promoter
     promoter        813..1177
                     /gene=SV40 early promoter
     polyA_signal    2022..2152
                     /gene=SV40 early polyA
     CDS             1208..2002
                     /gene=Neo(R)
     insertion_seq   280..331
                     /gene=shRNA
                     
BASE COUNT     1067 a      1028 c      1092 g      1054 t        42 others
ORIGIN
        1 cccgggacag aattcaaggt cgggcaggaa gagggcctat ttcccatgat tccttcatat 
       61 ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca 
      121 aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt 
      181 ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat 
      241 ttcttggctt tatatatctt gtggaaagga cgaaaaaaan nnnnnnnnnn nnnnnnnnnn 
      301 ttggatccaa nnnnnnnnnn nnnnnnnnnn ntttttttta agcttcacct tgtaagcgtt 
      361 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag 
      421 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 
      481 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 
      541 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 
      601 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 
      661 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 
      721 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 
      781 aatgcgccgc tacagggcgc gtcaggctcg agttctgagg cggaaagaac cagctgtgga 
      841 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 
      901 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 
      961 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 
     1021 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 
     1081 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 
     1141 gaggcttttt tggaggccta ggcttttgca aaaagctccc ggtaatacga ctcactatag 
     1201 ggctagcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag 
     1261 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg 
     1321 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa 
     1381 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc 
     1441 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc 
     1501 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga 
     1561 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa 
     1621 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct 
     1681 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat 
     1741 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt 
     1801 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta 
     1861 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga 
     1921 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg 
     1981 ccttcttgac gagttcttct gagcggccgc aatctagacc aaacttgttt attgcagctt 
     2041 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 
     2101 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtgatcagg 
     2161 taccaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 
     2221 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 
     2281 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 
     2341 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 
     2401 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 
     2461 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 
     2521 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 
     2581 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 
     2641 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 
     2701 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 
     2761 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 
     2821 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 
     2881 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 
     2941 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 
     3001 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 
     3061 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 
     3121 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 
     3181 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 
     3241 catatatact ttagattgat ttaaaacttc atttttaatt aaaaaggatc taggtgaaga 
     3301 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 
     3361 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 
     3421 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 
     3481 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 
     3541 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 
     3601 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 
     3661 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 
     3721 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 
     3781 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 
     3841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 
     3901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 
     3961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 
     4021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 
     4081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 
     4141 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 
     4201 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 
     4261 acgcaattaa tgtgagttag ctc 
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