LOCUS       pRNAi-H1-neo            4242 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     rep_origin      3351..3939
                     /gene=pUC ori
     CDS             2320..3180
                     /gene=Amp(R)
     rep_origin      309..765
                     /gene=f1 ori
     promoter        772..1136
                     /gene=SV40 early promoter
     polyA_signal    1981..2111
                     /gene=SV40 early polyA
     promoter        10..238
                     /gene=H1 promoter
     CDS             1167..1961
                     /gene=Neo(R)
     insertion_seq   239..290
                     /gene=shRNA
                     
BASE COUNT     1031 a      1048 c      1105 g      1016 t        42 others
ORIGIN
        1 cccgggacag aattcgaacg ctgacgtcat caacccgctc caaggaatcg cgggcccagt 
       61 gtcactaggc gggaacaccc agcgcgcgtg cgccctggca ggaagatggc tgtgagggac 
      121 aggggagtgg cgccctgcaa tatttgcatg tcgctatgtg ttctgggaaa tcaccataaa 
      181 cgtgaaatgt ctttggattt gggaatctta taagttctgt atgagaccac tctaaaaann 
      241 nnnnnnnnnn nnnnnnnnnt tggatccaan nnnnnnnnnn nnnnnnnnnn ttttttttaa 
      301 gcttcacctt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 
      361 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 
      421 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 
      481 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 
      541 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 
      601 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 
      661 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 
      721 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggctcga gttctgaggc 
      781 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 
      841 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 
      901 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 
      961 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 
     1021 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 
     1081 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg 
     1141 gtaatacgac tcactatagg gctagcatga ttgaacaaga tggattgcac gcaggttctc 
     1201 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 
     1261 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg 
     1321 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca 
     1381 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc 
     1441 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga 
     1501 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 
     1561 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 
     1621 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 
     1681 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct 
     1741 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc 
     1801 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 
     1861 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 
     1921 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcggccgca atctagacca 
     1981 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 
     2041 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 
     2101 tatcatgtct gtgatcaggt accaaagggc ctcgtgatac gcctattttt ataggttaat 
     2161 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga 
     2221 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 
     2281 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt 
     2341 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg 
     2401 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg 
     2461 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg 
     2521 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag 
     2581 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca 
     2641 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg 
     2701 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc 
     2761 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg 
     2821 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg 
     2881 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac 
     2941 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 
     3001 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg 
     3061 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 
     3121 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa 
     3181 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaatta 
     3241 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 
     3301 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 
     3361 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 
     3421 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 
     3481 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct 
     3541 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 
     3601 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 
     3661 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 
     3721 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 
     3781 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 
     3841 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 
     3901 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 
     3961 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 
     4021 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga 
     4081 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg 
     4141 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 
     4201 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tc 
//
