LOCUS       pRNAi-mU6-hyg           4565 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     rep_origin      3674..4262
                     /gene=pUC ori
     CDS             2643..3503
                     /gene=Amp(R)
     rep_origin      401..857
                     /gene=f1 ori
     promoter        864..1228
                     /gene=SV40 early promoter
     polyA_signal    2304..2434
                     /gene=SV40 early polyA
     CDS             1259..2284
                     /gene=Hyg(R)
     promoter        16..330
                     /gene=mouse U6 promoter
     insertion_seq   331..382
                     /gene=shRNA
                     
BASE COUNT     1143 a      1120 c      1162 g      1098 t        42 others
ORIGIN
        1 cccgggacag aattcgatcc gacgccgcca tctctaggcc cgcgccggcc ccctcgcaca 
       61 gacttgtggg agaagctcgg ctactcccct gccccggtta atttgcatat aatatttcct 
      121 agtaactata gaggcttaat gtgcgataaa agacagataa tctgttcttt ttaatactag 
      181 ctacatttta catgataggc ttggatttct ataagagata caaatactaa attattattt 
      241 taaaaaacag cacaaaagga aactcaccct aactgtaaag taattgtgtg ttttgagact 
      301 ataaatatcc cttggagaaa agcctaaaaa nnnnnnnnnn nnnnnnnnnn nttggatcca 
      361 annnnnnnnn nnnnnnnnnn nntttttttt aagcttcacc ttgtaagcgt taatattttg 
      421 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc 
      481 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt 
      541 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 
      601 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg 
      661 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga 
      721 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 
      781 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg 
      841 ctacagggcg cgtcaggctc gagttctgag gcggaaagaa ccagctgtgg aatgtgtgtc 
      901 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 
      961 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 
     1021 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 
     1081 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 
     1141 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 
     1201 ttggaggcct aggcttttgc aaaaagctcc cggtaatacg actcactata gggctagcat 
     1261 gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag 
     1321 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt 
     1381 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg 
     1441 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg 
     1501 ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca 
     1561 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc 
     1621 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat 
     1681 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca 
     1741 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct 
     1801 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc 
     1861 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat 
     1921 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg 
     1981 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg 
     2041 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg 
     2101 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc 
     2161 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg 
     2221 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga 
     2281 ataggcggcc gcaatctaga ccaaacttgt ttattgcagc ttataatggt tacaaataaa 
     2341 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 
     2401 tgtccaaact catcaatgta tcttatcatg tctgtgatca ggtaccaaag ggcctcgtga 
     2461 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca 
     2521 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 
     2581 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 
     2641 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 
     2701 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 
     2761 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 
     2821 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 
     2881 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 
     2941 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 
     3001 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 
     3061 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 
     3121 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 
     3181 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 
     3241 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 
     3301 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 
     3361 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 
     3421 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 
     3481 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 
     3541 atttaaaact tcatttttaa ttaaaaagga tctaggtgaa gatccttttt gataatctca 
     3601 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 
     3661 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 
     3721 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 
     3781 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt 
     3841 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 
     3901 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 
     3961 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 
     4021 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 
     4081 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 
     4141 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 
     4201 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 
     4261 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 
     4321 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 
     4381 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 
     4441 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 
     4501 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt 
     4561 agctc 
//
