LOCUS       pRNAi-hU6-hyg           4514 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     rep_origin      3623..4211
                     /gene=pUC ori
     CDS             2592..3452
                     /gene=Amp(R)
     rep_origin      350..806
                     /gene=f1 ori
     promoter        16..279
                     /gene=human U6 promoter
     promoter        813..1177
                     /gene=SV40 early promoter
     polyA_signal    2253..2383
                     /gene=SV40 early polyA
     CDS             1208..2233
                     /gene=Hyg(R)
     insertion_seq   280..331
                     /gene=shRNA
BASE COUNT     1133 a      1085 c      1158 g      1096 t        42 others
ORIGIN
        1 cccgggacag aattcaaggt cgggcaggaa gagggcctat ttcccatgat tccttcatat 
       61 ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca 
      121 aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt 
      181 ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat 
      241 ttcttggctt tatatatctt gtggaaagga cgaaaaaaan nnnnnnnnnn nnnnnnnnnn 
      301 ttggatccaa nnnnnnnnnn nnnnnnnnnn ntttttttta agcttcacct tgtaagcgtt 
      361 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag 
      421 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 
      481 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 
      541 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 
      601 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 
      661 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 
      721 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 
      781 aatgcgccgc tacagggcgc gtcaggctcg agttctgagg cggaaagaac cagctgtgga 
      841 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 
      901 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 
      961 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 
     1021 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 
     1081 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 
     1141 gaggcttttt tggaggccta ggcttttgca aaaagctccc ggtaatacga ctcactatag 
     1201 ggctagcatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa 
     1261 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag 
     1321 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta 
     1381 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct 
     1441 tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt 
     1501 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc 
     1561 catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc 
     1621 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca 
     1681 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct 
     1741 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga 
     1801 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag 
     1861 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg 
     1921 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg 
     1981 atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt 
     2041 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg 
     2101 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac 
     2161 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag 
     2221 ggcaaaggaa taggcggccg caatctagac caaacttgtt tattgcagct tataatggtt 
     2281 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 
     2341 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtgatcag gtaccaaagg 
     2401 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt 
     2461 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 
     2521 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 
     2581 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 
     2641 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 
     2701 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 
     2761 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 
     2821 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 
     2881 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 
     2941 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 
     3001 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 
     3061 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 
     3121 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 
     3181 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 
     3241 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 
     3301 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 
     3361 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 
     3421 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 
     3481 tttagattga tttaaaactt catttttaat taaaaaggat ctaggtgaag atcctttttg 
     3541 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 
     3601 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 
     3661 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 
     3721 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt 
     3781 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 
     3841 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 
     3901 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 
     3961 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 
     4021 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 
     4081 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 
     4141 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 
     4201 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 
     4261 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 
     4321 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 
     4381 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 
     4441 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 
     4501 atgtgagtta gctc 
//
