LOCUS       pRNAi-H1-hyg            4473 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     rep_origin      3582..4170
                     /gene=pUC ori
     CDS             2551..3411
                     /gene=Amp(R)
     rep_origin      309..765
                     /gene=f1 ori
     promoter        772..1136
                     /gene=SV40 early promoter
     polyA_signal    2212..2342
                     /gene=SV40 early polyA
     CDS             1167..2192
                     /gene=Hyg(R)
     promoter        10..238
                     /gene=H1 promoter
     insertion_seq   239..290
                     /gene=shRNA
                     
BASE COUNT     1097 a      1105 c      1171 g      1058 t        42 others
ORIGIN
        1 cccgggacag aattcgaacg ctgacgtcat caacccgctc caaggaatcg cgggcccagt 
       61 gtcactaggc gggaacaccc agcgcgcgtg cgccctggca ggaagatggc tgtgagggac 
      121 aggggagtgg cgccctgcaa tatttgcatg tcgctatgtg ttctgggaaa tcaccataaa 
      181 cgtgaaatgt ctttggattt gggaatctta taagttctgt atgagaccac tctaaaaann 
      241 nnnnnnnnnn nnnnnnnnnt tggatccaan nnnnnnnnnn nnnnnnnnnn ttttttttaa 
      301 gcttcacctt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 
      361 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 
      421 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 
      481 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 
      541 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 
      601 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 
      661 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 
      721 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggctcga gttctgaggc 
      781 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 
      841 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 
      901 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 
      961 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 
     1021 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 
     1081 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg 
     1141 gtaatacgac tcactatagg gctagcatga aaaagcctga actcaccgcg acgtctgtcg 
     1201 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg 
     1261 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata 
     1321 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc 
     1381 tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct 
     1441 cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc 
     1501 tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg 
     1561 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat 
     1621 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg 
     1681 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc 
     1741 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa 
     1801 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca 
     1861 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga 
     1921 ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg 
     1981 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc 
     2041 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca 
     2101 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac 
     2161 gccccagcac tcgtccgagg gcaaaggaat aggcggccgc aatctagacc aaacttgttt 
     2221 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 
     2281 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 
     2341 tgtgatcagg taccaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata 
     2401 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt 
     2461 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 
     2521 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt 
     2581 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa 
     2641 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac 
     2701 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt 
     2761 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt 
     2821 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat 
     2881 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac 
     2941 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg 
     3001 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc 
     3061 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa 
     3121 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag 
     3181 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct 
     3241 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat 
     3301 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa 
     3361 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac 
     3421 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt aaaaaggatc 
     3481 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 
     3541 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 
     3601 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 
     3661 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 
     3721 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 
     3781 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 
     3841 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 
     3901 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 
     3961 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 
     4021 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 
     4081 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 
     4141 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 
     4201 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 
     4261 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag 
     4321 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc 
     4381 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc 
     4441 agtgagcgca acgcaattaa tgtgagttag ctc 
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