LOCUS       pRNAi-mU6-puro          4143 bp    DNA     circular     22-JUN-2008

FEATURES             Location/Qualifiers
     rep_origin      3252..3840
                     /gene=pUC ori
     CDS             2221..3081
                     /gene=Amp(R)
     rep_origin      401..857
                     /gene=f1 ori
     promoter        864..1228
                     /gene=SV40 early promoter
     polyA_signal    1882..2012
                     /gene=SV40 early polyA
     promoter        16..330
                     /gene=mouse U6 promoter
     insertion_seq   331..382
                     /gene=shRNA
     CDS             1263..1862
                     /gene=Puro(R)
                     
BASE COUNT     1020 a      1075 c      1052 g       954 t        42 others
ORIGIN
        1 cccgggacag aattcgatcc gacgccgcca tctctaggcc cgcgccggcc ccctcgcaca 
       61 gacttgtggg agaagctcgg ctactcccct gccccggtta atttgcatat aatatttcct 
      121 agtaactata gaggcttaat gtgcgataaa agacagataa tctgttcttt ttaatactag 
      181 ctacatttta catgataggc ttggatttct ataagagata caaatactaa attattattt 
      241 taaaaaacag cacaaaagga aactcaccct aactgtaaag taattgtgtg ttttgagact 
      301 ataaatatcc cttggagaaa agcctaaaaa nnnnnnnnnn nnnnnnnnnn nttggatcca 
      361 annnnnnnnn nnnnnnnnnn nntttttttt aagcttcacc ttgtaagcgt taatattttg 
      421 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc 
      481 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt 
      541 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 
      601 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg 
      661 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga 
      721 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 
      781 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg 
      841 ctacagggcg cgtcaggctc gagttctgag gcggaaagaa ccagctgtgg aatgtgtgtc 
      901 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 
      961 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 
     1021 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 
     1081 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 
     1141 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 
     1201 ttggaggcct aggcttttgc aaaaagctcc cggtaatacg actcactata gggctagcca 
     1261 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg 
     1321 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg 
     1381 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg 
     1441 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg 
     1501 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg 
     1561 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg 
     1621 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg 
     1681 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc 
     1741 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg 
     1801 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct 
     1861 gagcggccgc aatctagacc aaacttgttt attgcagctt ataatggtta caaataaagc 
     1921 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 
     1981 tccaaactca tcaatgtatc ttatcatgtc tgtgatcagg taccaaaggg cctcgtgata 
     2041 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact 
     2101 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 
     2161 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 
     2221 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct 
     2281 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca 
     2341 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc 
     2401 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc 
     2461 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg 
     2521 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta 
     2581 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc 
     2641 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt 
     2701 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg 
     2761 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct 
     2821 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc 
     2881 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct 
     2941 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac 
     3001 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc 
     3061 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat 
     3121 ttaaaacttc atttttaatt aaaaaggatc taggtgaaga tcctttttga taatctcatg 
     3181 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 
     3241 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 
     3301 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 
     3361 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta 
     3421 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 
     3481 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 
     3541 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 
     3601 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 
     3661 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 
     3721 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 
     3781 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 
     3841 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 
     3901 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct 
     3961 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa 
     4021 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg 
     4081 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag 
     4141 ctc 
//
