LOCUS       pRNAi-hU6-puro          4092 bp    DNA     circular     22-JUN-2008

FEATURES             Location/Qualifiers
     rep_origin      3201..3789
                     /gene=pUC ori
     CDS             2170..3030
                     /gene=Amp(R)
     rep_origin      350..806
                     /gene=f1 ori
     promoter        16..279
                     /gene=human U6 promoter
     promoter        813..1177
                     /gene=SV40 early promoter
     polyA_signal    1831..1961
                     /gene=SV40 early polyA
     insertion_seq   280..331
                     /gene=shRNA
     CDS             1212..1811
                     /gene=Puro(R)
                     
BASE COUNT     1010 a      1040 c      1048 g       952 t        42 others
ORIGIN
        1 cccgggacag aattcaaggt cgggcaggaa gagggcctat ttcccatgat tccttcatat 
       61 ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca 
      121 aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt 
      181 ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat 
      241 ttcttggctt tatatatctt gtggaaagga cgaaaaaaan nnnnnnnnnn nnnnnnnnnn 
      301 ttggatccaa nnnnnnnnnn nnnnnnnnnn ntttttttta agcttcacct tgtaagcgtt 
      361 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag 
      421 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 
      481 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 
      541 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 
      601 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 
      661 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 
      721 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 
      781 aatgcgccgc tacagggcgc gtcaggctcg agttctgagg cggaaagaac cagctgtgga 
      841 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 
      901 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 
      961 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 
     1021 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 
     1081 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 
     1141 gaggcttttt tggaggccta ggcttttgca aaaagctccc ggtaatacga ctcactatag 
     1201 ggctagccac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc 
     1261 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg 
     1321 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg 
     1381 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga 
     1441 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg 
     1501 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc 
     1561 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca 
     1621 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc 
     1681 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca 
     1741 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc 
     1801 ccggtgcctg agcggccgca atctagacca aacttgttta ttgcagctta taatggttac 
     1861 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 
     1921 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtgatcaggt accaaagggc 
     1981 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca 
     2041 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 
     2101 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 
     2161 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 
     2221 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 
     2281 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 
     2341 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 
     2401 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 
     2461 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 
     2521 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 
     2581 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 
     2641 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 
     2701 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 
     2761 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 
     2821 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 
     2881 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 
     2941 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 
     3001 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 
     3061 tagattgatt taaaacttca tttttaatta aaaaggatct aggtgaagat cctttttgat 
     3121 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 
     3181 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 
     3241 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 
     3301 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 
     3361 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 
     3421 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 
     3481 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 
     3541 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 
     3601 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 
     3661 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 
     3721 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 
     3781 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 
     3841 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 
     3901 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 
     3961 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 
     4021 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 
     4081 gtgagttagc tc 
//
