LOCUS       pRNAi-H1-puro           4051 bp    DNA     circular     22-JUN-2008

FEATURES             Location/Qualifiers
     rep_origin      3160..3748
                     /gene=pUC ori
     CDS             2129..2989
                     /gene=Amp(R)
     rep_origin      309..765
                     /gene=f1 ori
     promoter        772..1136
                     /gene=SV40 early promoter
     polyA_signal    1790..1920
                     /gene=SV40 early polyA
     promoter        10..238
                     /gene=H1 promoter
     insertion_seq   239..290
                     /gene=shRNA
     CDS             1171..1770
                     /gene=Puro(R)
                     
BASE COUNT      974 a      1060 c      1061 g       914 t        42 others
ORIGIN
        1 cccgggacag aattcgaacg ctgacgtcat caacccgctc caaggaatcg cgggcccagt 
       61 gtcactaggc gggaacaccc agcgcgcgtg cgccctggca ggaagatggc tgtgagggac 
      121 aggggagtgg cgccctgcaa tatttgcatg tcgctatgtg ttctgggaaa tcaccataaa 
      181 cgtgaaatgt ctttggattt gggaatctta taagttctgt atgagaccac tctaaaaann 
      241 nnnnnnnnnn nnnnnnnnnt tggatccaan nnnnnnnnnn nnnnnnnnnn ttttttttaa 
      301 gcttcacctt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 
      361 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 
      421 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 
      481 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 
      541 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 
      601 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 
      661 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 
      721 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggctcga gttctgaggc 
      781 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 
      841 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 
      901 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 
      961 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 
     1021 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 
     1081 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg 
     1141 gtaatacgac tcactatagg gctagccacc atgaccgagt acaagcccac ggtgcgcctc 
     1201 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 
     1261 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 
     1321 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 
     1381 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 
     1441 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 
     1501 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 
     1561 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 
     1621 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 
     1681 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 
     1741 tggtgcatga cccgcaagcc cggtgcctga gcggccgcaa tctagaccaa acttgtttat 
     1801 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 
     1861 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 
     1921 tgatcaggta ccaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat 
     1981 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 
     2041 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 
     2101 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 
     2161 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 
     2221 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 
     2281 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 
     2341 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg 
     2401 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 
     2461 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 
     2521 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 
     2581 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 
     2641 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 
     2701 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 
     2761 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 
     2821 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 
     2881 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 
     2941 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 
     3001 agtttactca tatatacttt agattgattt aaaacttcat ttttaattaa aaaggatcta 
     3061 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 
     3121 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 
     3181 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 
     3241 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 
     3301 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 
     3361 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 
     3421 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 
     3481 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 
     3541 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 
     3601 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 
     3661 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 
     3721 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 
     3781 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 
     3841 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 
     3901 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc 
     3961 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag 
     4021 tgagcgcaac gcaattaatg tgagttagct c 
//
