LOCUS       pLV-H1TetO-RFP-Bsd     10074 bp    DNA     circular     19-MAR-1980
SOURCE      
COMMENT     
            This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     CDS             7995..8855
                     /label=Amp(R)
     promoter        9056..9596
                     /label=pUC\ori
     promoter        7..235
                     /label=RSV
     LTR             6600..6834
                     /label=3'\LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(7409..7864)
                     /label=f1\ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6906..7036
                     /label=SV40\pA
     misc_feature    7037..7250
                     /label=SV40\ori
     promoter        2104..2499
                     /label=human\EF1a\promoter
     intron          2532..3474
                     /label=intron
     promoter        complement(1872..1971)
                     /label=H1TetO
     CDS             3531..4172
                     /label=TetR
     RBS             4176..4177
                     /label=IRES
     stem_loop       complement(1824..1871)
                     /label=shRNA
     RBS             4176..4760
                     /label=IRES
     CDS             4787..5911
                     /label=RFP-Bsd
BASE COUNT     2493 a      2491 c      2637 g      2415 t        38 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnttggatcc aannnnnnnn 
     1861 nnnnnnnnnn ntttttctct atcactgata gggaacttat aagtctctat cactgatagg 
     1921 gatttcacgt ttatggtgat ttcccagaac acatagcgac atgcaaatat tggatccaac 
     1981 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat 
     2041 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc 
     2101 gatgcctccc cgtcaccacc ccccccaacc cgccccgacc ggagctgaga gtaattcata 
     2161 caaaaggact cgcccctgcc ttggggaatc ccagggaccg tcgttaaact cccactaacg 
     2221 tagaacccag agatcgctgc gttcccgccc cctcacccgc ccgctctcgt catcactgag 
     2281 gtggagaaga gcatgcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca 
     2341 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc 
     2401 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 
     2461 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 
     2521 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg 
     2581 gcccttgcgt gccttgaatt acttccacgc ccctggctgc agtacgtgat tcttgatccc 
     2641 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg 
     2701 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg 
     2761 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga 
     2821 tatcctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac 
     2881 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca 
     2941 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa 
     3001 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg 
     3061 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct 
     3121 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc 
     3181 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac 
     3241 cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt 
     3301 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt 
     3361 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct 
     3421 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg 
     3481 tgaaaactac ccctctagat aatacgactc actatagggc ccgggccacc atgtccagat 
     3541 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag 
     3601 gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc 
     3661 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc 
     3721 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta 
     3781 aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac 
     3841 ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag 
     3901 gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt 
     3961 gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg 
     4021 atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc 
     4081 cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg 
     4141 aaagtgggtc cgcgtacagc ggctcccggt agctagcccc tctccctccc ccccccctaa 
     4201 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc 
     4261 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac 
     4321 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt 
     4381 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg 
     4441 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata 
     4501 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga 
     4561 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt 
     4621 accccattgt atgggatctg atctggggcc tcggtacaca tgctttacat gtgtttagtc 
     4681 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac 
     4741 acgatgataa tatggccaca ctagagtcga gctaccggtc gccaccatgg tgagcaaggg 
     4801 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg 
     4861 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg 
     4921 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat 
     4981 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc 
     5041 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga 
     5101 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta 
     5161 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac 
     5221 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga 
     5281 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac 
     5341 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga 
     5401 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg 
     5461 ccactccacc ggcggcatgg acgagctgta caaggaccca ccggtcgcca ccatggccaa 
     5521 gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat 
     5581 ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt 
     5641 cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg 
     5701 cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa 
     5761 caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg 
     5821 gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt 
     5881 gctgccctct ggttatgtgt gggagggcta agcggccgcg tcgacaatca acctctggat 
     5941 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 
     6001 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 
     6061 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 
     6121 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 
     6181 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 
     6241 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 
     6301 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc 
     6361 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 
     6421 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag 
     6481 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttcgagctcg gtacctttaa 
     6541 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac 
     6601 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc 
     6661 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 
     6721 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 
     6781 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 
     6841 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 
     6901 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 
     6961 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 
     7021 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac 
     7081 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 
     7141 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 
     7201 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc 
     7261 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca 
     7321 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa 
     7381 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg 
     7441 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 
     7501 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 
     7561 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 
     7621 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 
     7681 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 
     7741 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 
     7801 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca 
     7861 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 
     7921 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 
     7981 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg 
     8041 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag 
     8101 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg 
     8161 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg 
     8221 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt 
     8281 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga 
     8341 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac 
     8401 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc 
     8461 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc 
     8521 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac 
     8581 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag 
     8641 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 
     8701 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 
     8761 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg 
     8821 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 
     8881 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 
     8941 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 
     9001 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 
     9061 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 
     9121 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 
     9181 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 
     9241 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 
     9301 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 
     9361 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 
     9421 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 
     9481 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 
     9541 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 
     9601 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 
     9661 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 
     9721 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 
     9781 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 
     9841 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 
     9901 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc 
     9961 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg 
    10021 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgc 
//
