LOCUS       pLV-H1TetO-GFP-Bsd   10080 bp    DNA     circular     31-MAR-2010
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     CDS             8001..8861
                     /label=Amp(R)
     promoter        9062..9602
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             6606..6840
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(7415..7870)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6912..7042
                     /label=SV40 pA
     misc_feature    7043..7256
                     /label=SV40 ori
     promoter        2104..2499
                     /label=human EF1a promoter
     intron          2532..3474
                     /label=intron
     promoter        complement(1872..1971)
                     /label=H1TetO
     CDS             3531..4172
                     /label=TetR
     RBS             4176..4760
                     /label=IRES
     CDS             4784..5917
                     /label=GFP-Bsd
     stem_loop       complement(1824..1871)
                     /label=shRNA
BASE COUNT     2502 a      2506 c      2618 g      2416 t        38 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnttggatcc aannnnnnnn 
     1861 nnnnnnnnnn ntttttctct atcactgata gggaacttat aagtctctat cactgatagg 
     1921 gatttcacgt ttatggtgat ttcccagaac acatagcgac atgcaaatat tggatccaac 
     1981 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat 
     2041 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc 
     2101 gatgcctccc cgtcaccacc ccccccaacc cgccccgacc ggagctgaga gtaattcata 
     2161 caaaaggact cgcccctgcc ttggggaatc ccagggaccg tcgttaaact cccactaacg 
     2221 tagaacccag agatcgctgc gttcccgccc cctcacccgc ccgctctcgt catcactgag 
     2281 gtggagaaga gcatgcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca 
     2341 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc 
     2401 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 
     2461 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 
     2521 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg 
     2581 gcccttgcgt gccttgaatt acttccacgc ccctggctgc agtacgtgat tcttgatccc 
     2641 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg 
     2701 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg 
     2761 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga 
     2821 tatcctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac 
     2881 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca 
     2941 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa 
     3001 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg 
     3061 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct 
     3121 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc 
     3181 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac 
     3241 cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt 
     3301 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt 
     3361 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct 
     3421 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg 
     3481 tgaaaactac ccctctagat aatacgactc actatagggc ccgggccacc atgtccagat 
     3541 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag 
     3601 gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc 
     3661 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc 
     3721 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta 
     3781 aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac 
     3841 ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag 
     3901 gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt 
     3961 gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg 
     4021 atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc 
     4081 cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg 
     4141 aaagtgggtc cgcgtacagc ggctcccggt agctagcccc tctccctccc ccccccctaa 
     4201 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc 
     4261 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac 
     4321 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt 
     4381 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg 
     4441 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata 
     4501 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga 
     4561 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt 
     4621 accccattgt atgggatctg atctggggcc tcggtacaca tgctttacat gtgtttagtc 
     4681 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac 
     4741 acgatgataa tatggccaca ctagagatcc accggtcgcc accatggtga gcaagggcga 
     4801 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca 
     4861 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa 
     4921 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac 
     4981 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa 
     5041 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa 
     5101 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct 
     5161 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta 
     5221 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt 
     5281 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa 
     5341 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc 
     5401 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac 
     5461 cgccgccggg atcactctcg gcatggacga gctgtacaag gacccaccgg tcgccaccat 
     5521 ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa 
     5581 cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg 
     5641 catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt 
     5701 gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa 
     5761 tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca 
     5821 tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg 
     5881 tgaattgctg ccctctggtt atgtgtggga gggctaagcg gccgcgtcga caatcaacct 
     5941 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 
     6001 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 
     6061 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 
     6121 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 
     6181 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 
     6241 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 
     6301 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt 
     6361 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 
     6421 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc 
     6481 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattcg agctcggtac 
     6541 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg 
     6601 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg 
     6661 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 
     6721 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 
     6781 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 
     6841 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag 
     6901 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 
     6961 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 
     7021 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc 
     7081 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 
     7141 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 
     7201 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg 
     7261 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt 
     7321 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct 
     7381 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc 
     7441 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 
     7501 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 
     7561 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 
     7621 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 
     7681 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 
     7741 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 
     7801 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg 
     7861 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 
     7921 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 
     7981 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 
     8041 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 
     8101 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 
     8161 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 
     8221 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 
     8281 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 
     8341 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 
     8401 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 
     8461 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 
     8521 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 
     8581 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 
     8641 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 
     8701 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 
     8761 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 
     8821 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 
     8881 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 
     8941 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 
     9001 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 
     9061 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 
     9121 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 
     9181 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 
     9241 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 
     9301 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 
     9361 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 
     9421 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 
     9481 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 
     9541 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 
     9601 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 
     9661 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 
     9721 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 
     9781 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 
     9841 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 
     9901 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 
     9961 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 
    10021 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc 
//
