LOCUS       pLV-H1TetO-RFP-Puro   10275 bp    DNA     circular     31-MAR-2010
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     CDS             8196..9056
                     /label=Amp(R)
     promoter        9257..9797
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             6801..7035
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(7610..8065)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    7107..7237
                     /label=SV40 pA
     misc_feature    7238..7451
                     /label=SV40 ori
     promoter        2104..2499
                     /label=human EF1a promoter
     intron          2532..3474
                     /label=intron
     promoter        complement(1872..1971)
                     /label=H1TetO
     CDS             3531..4172
                     /label=TetR
     RBS             4176..4760
                     /label=IRES
     CDS             4787..6112
                     /label=RFP-Puro
     stem_loop       complement(1824..1871)
                     /label=shRNA
BASE COUNT     2491 a      2619 c      2728 g      2399 t        38 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnttggatcc aannnnnnnn 
     1861 nnnnnnnnnn ntttttctct atcactgata gggaacttat aagtctctat cactgatagg 
     1921 gatttcacgt ttatggtgat ttcccagaac acatagcgac atgcaaatat tggatccaac 
     1981 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat 
     2041 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc 
     2101 gatgcctccc cgtcaccacc ccccccaacc cgccccgacc ggagctgaga gtaattcata 
     2161 caaaaggact cgcccctgcc ttggggaatc ccagggaccg tcgttaaact cccactaacg 
     2221 tagaacccag agatcgctgc gttcccgccc cctcacccgc ccgctctcgt catcactgag 
     2281 gtggagaaga gcatgcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca 
     2341 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc 
     2401 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 
     2461 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 
     2521 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg 
     2581 gcccttgcgt gccttgaatt acttccacgc ccctggctgc agtacgtgat tcttgatccc 
     2641 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg 
     2701 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg 
     2761 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga 
     2821 tatcctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac 
     2881 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca 
     2941 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa 
     3001 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg 
     3061 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct 
     3121 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc 
     3181 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac 
     3241 cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt 
     3301 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt 
     3361 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct 
     3421 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg 
     3481 tgaaaactac ccctctagat aatacgactc actatagggc ccgggccacc atgtccagat 
     3541 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag 
     3601 gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc 
     3661 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc 
     3721 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta 
     3781 aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac 
     3841 ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag 
     3901 gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt 
     3961 gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg 
     4021 atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc 
     4081 cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg 
     4141 aaagtgggtc cgcgtacagc ggctcccggt agctagcccc tctccctccc ccccccctaa 
     4201 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc 
     4261 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac 
     4321 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt 
     4381 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg 
     4441 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata 
     4501 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga 
     4561 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt 
     4621 accccattgt atgggatctg atctggggcc tcggtacaca tgctttacat gtgtttagtc 
     4681 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac 
     4741 acgatgataa tatggccaca ctagagtcga gctaccggtc gccaccatgg tgagcaaggg 
     4801 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg 
     4861 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg 
     4921 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat 
     4981 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc 
     5041 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga 
     5101 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta 
     5161 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac 
     5221 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga 
     5281 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac 
     5341 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga 
     5401 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg 
     5461 ccactccacc ggcggcatgg acgagctgta caaggaccca ccggtcgcca ccatgaccga 
     5521 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct 
     5581 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat 
     5641 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 
     5701 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 
     5761 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct 
     5821 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg 
     5881 gttcctggcc accgtcggcg tgtcgcccga ccaccagggc aagggtctgg gcagcgccgt 
     5941 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc 
     6001 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 
     6061 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gagcggccgc 
     6121 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 
     6181 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 
     6241 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 
     6301 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 
     6361 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 
     6421 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 
     6481 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg 
     6541 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 
     6601 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 
     6661 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga 
     6721 attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact 
     6781 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc 
     6841 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct 
     6901 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt 
     6961 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt 
     7021 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa 
     7081 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat 
     7141 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 
     7201 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac 
     7261 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 
     7321 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta 
     7381 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat 
     7441 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc 
     7501 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc 
     7561 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt 
     7621 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 
     7681 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 
     7741 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 
     7801 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 
     7861 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 
     7921 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 
     7981 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt 
     8041 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc 
     8101 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 
     8161 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 
     8221 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 
     8281 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 
     8341 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 
     8401 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 
     8461 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 
     8521 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 
     8581 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 
     8641 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 
     8701 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 
     8761 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 
     8821 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 
     8881 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 
     8941 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 
     9001 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 
     9061 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 
     9121 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 
     9181 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 
     9241 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 
     9301 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 
     9361 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 
     9421 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 
     9481 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 
     9541 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 
     9601 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 
     9661 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 
     9721 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 
     9781 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 
     9841 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 
     9901 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 
     9961 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc 
    10021 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 
    10081 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 
    10141 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca 
    10201 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac 
    10261 aaaagctgga gctgc 
//
