LOCUS       pLV-H1TetO-GFP-Puro  10281 bp    DNA     circular     25-SEP-2010
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     CDS             8202..9062
                     /label=Amp(R)
     promoter        9263..9803
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             6807..7041
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(7616..8071)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    7113..7243
                     /label=SV40 pA
     misc_feature    7244..7457
                     /label=SV40 ori
     promoter        2104..2499
                     /label=human EF1a promoter
     intron          2532..3474
                     /label=intron
     promoter        complement(1872..1971)
                     /label=H1TetO
     CDS             3531..4172
                     /label=TetR
     RBS             4176..4760
                     /label=IRES
     CDS             4784..6118
                     /label=GFP-Puro
     stem_loop       complement(1824..1871)
                     /label=shRNA
BASE COUNT     2500 a      2634 c      2709 g      2400 t        38 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnttggatcc aannnnnnnn 
     1861 nnnnnnnnnn ntttttctct atcactgata gggaacttat aagtctctat cactgatagg 
     1921 gatttcacgt ttatggtgat ttcccagaac acatagcgac atgcaaatat tggatccaac 
     1981 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat 
     2041 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc 
     2101 gatgcctccc cgtcaccacc ccccccaacc cgccccgacc ggagctgaga gtaattcata 
     2161 caaaaggact cgcccctgcc ttggggaatc ccagggaccg tcgttaaact cccactaacg 
     2221 tagaacccag agatcgctgc gttcccgccc cctcacccgc ccgctctcgt catcactgag 
     2281 gtggagaaga gcatgcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca 
     2341 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc 
     2401 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 
     2461 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 
     2521 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg 
     2581 gcccttgcgt gccttgaatt acttccacgc ccctggctgc agtacgtgat tcttgatccc 
     2641 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg 
     2701 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg 
     2761 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga 
     2821 tatcctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac 
     2881 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca 
     2941 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa 
     3001 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg 
     3061 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct 
     3121 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc 
     3181 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac 
     3241 cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt 
     3301 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt 
     3361 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct 
     3421 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg 
     3481 tgaaaactac ccctctagat aatacgactc actatagggc ccgggccacc atgtccagat 
     3541 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag 
     3601 gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc 
     3661 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc 
     3721 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta 
     3781 aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac 
     3841 ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag 
     3901 gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt 
     3961 gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg 
     4021 atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc 
     4081 cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg 
     4141 aaagtgggtc cgcgtacagc ggctcccggt agctagcccc tctccctccc ccccccctaa 
     4201 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc 
     4261 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac 
     4321 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt 
     4381 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg 
     4441 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata 
     4501 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga 
     4561 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt 
     4621 accccattgt atgggatctg atctggggcc tcggtacaca tgctttacat gtgtttagtc 
     4681 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac 
     4741 acgatgataa tatggccaca ctagagatcc accggtcgcc accatggtga gcaagggcga 
     4801 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca 
     4861 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa 
     4921 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac 
     4981 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa 
     5041 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa 
     5101 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct 
     5161 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta 
     5221 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt 
     5281 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa 
     5341 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc 
     5401 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac 
     5461 cgccgccggg atcactctcg gcatggacga gctgtacaag gacccaccgg tcgccaccat 
     5521 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg 
     5581 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg 
     5641 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 
     5701 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 
     5761 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 
     5821 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 
     5881 cgcgtggttc ctggccaccg tcggcgtgtc gcccgaccac cagggcaagg gtctgggcag 
     5941 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 
     6001 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 
     6061 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagc 
     6121 ggccgcgtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 
     6181 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 
     6241 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 
     6301 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 
     6361 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 
     6421 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 
     6481 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt 
     6541 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 
     6601 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 
     6661 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg 
     6721 cctggaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta 
     6781 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag 
     6841 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct 
     6901 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 
     6961 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 
     7021 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac 
     7081 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt 
     7141 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 
     7201 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc 
     7261 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 
     7321 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 
     7381 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag 
     7441 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc 
     7501 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa 
     7561 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg 
     7621 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 
     7681 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 
     7741 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 
     7801 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 
     7861 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 
     7921 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 
     7981 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 
     8041 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg 
     8101 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 
     8161 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc 
     8221 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 
     8281 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac 
     8341 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga 
     8401 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag 
     8461 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca 
     8521 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca 
     8581 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa 
     8641 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc 
     8701 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa 
     8761 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag 
     8821 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct 
     8881 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac 
     8941 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa 
     9001 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt 
     9061 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat 
     9121 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 
     9181 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 
     9241 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 
     9301 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 
     9361 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact 
     9421 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 
     9481 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 
     9541 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 
     9601 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 
     9661 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 
     9721 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 
     9781 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 
     9841 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 
     9901 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc 
     9961 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac 
    10021 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact 
    10081 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc 
    10141 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat 
    10201 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa 
    10261 gggaacaaaa gctggagctg c 
//
