LOCUS       pLV-hU6-EF1a-RFP-Puro   9183 bp    DNA     circular     26-SEP-2010
SOURCE      
COMMENT     
            This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     CDS             7104..7964
                     /label=Amp(R)
     promoter        8165..8705
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5709..5943
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6518..6973)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6015..6145
                     /label=SV40 pA
     misc_feature    6146..6359
                     /label=SV40 ori
     promoter        2278..2673
                     /label=human EF1a promoter
     intron          2706..3648
                     /label=intron
     promoter        complement(1872..2135)
                     /label=human U6
     CDS             3695..5020
                     /label=RFP-Puro
     stem_loop       complement(1824..1871)
                     /label=shRNA
BASE COUNT     2199 a      2361 c      2443 g      2142 t        38 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnttggatcc aannnnnnnn 
     1861 nnnnnnnnnn ntttttttcg tcctttccac aagatatata aagccaagaa atcgaaatac 
     1921 tttcaagtta cggtaagcat atgatagtcc attttaaaac ataattttaa aactgcaaac 
     1981 tacccaagaa attattactt tctacgtcac gtattttgta ctaatatctt tgtgtttaca 
     2041 gtcaaattaa ttctaattat ctctctaaca gccttgtatc gtatatgcaa atatgaagga 
     2101 atcatgggaa ataggccctc ttcctgcccg accttgaatt cggccggatc caacttttaa 
     2161 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac 
     2221 agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgatgcc 
     2281 tccccgtcac cacccccccc aacccgcccc gaccggagct gagagtaatt catacaaaag 
     2341 gactcgcccc tgccttgggg aatcccaggg accgtcgtta aactcccact aacgtagaac 
     2401 ccagagatcg ctgcgttccc gccccctcac ccgcccgctc tcgtcatcac tgaggtggag 
     2461 aagagcatgc gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc 
     2521 ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg 
     2581 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac 
     2641 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa 
     2701 cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt 
     2761 gcgtgccttg aattacttcc acgcccctgg ctgcagtacg tgattcttga tcccgagctt 
     2821 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt 
     2881 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt 
     2941 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgatatcct 
     3001 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt 
     3061 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg 
     3121 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc 
     3181 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg 
     3241 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca 
     3301 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa 
     3361 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg 
     3421 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg 
     3481 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca 
     3541 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc 
     3601 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgaaaa 
     3661 ctacccctct agagtcgagc taccggtcgc caccatggtg agcaagggcg aggaggataa 
     3721 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg 
     3781 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 
     3841 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca 
     3901 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa 
     3961 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt 
     4021 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct 
     4081 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga 
     4141 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag 
     4201 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa 
     4261 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca 
     4321 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg 
     4381 cggcatggac gagctgtaca aggacccacc ggtcgccacc atgaccgagt acaagcccac 
     4441 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt 
     4501 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac 
     4561 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc 
     4621 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt 
     4681 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca 
     4741 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac 
     4801 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg 
     4861 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa 
     4921 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg 
     4981 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga gcggccgcgt cgacaatcaa 
     5041 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 
     5101 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 
     5161 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 
     5221 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 
     5281 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 
     5341 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 
     5401 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt 
     5461 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 
     5521 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt 
     5581 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat tcgagctcgg 
     5641 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa 
     5701 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta 
     5761 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 
     5821 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 
     5881 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 
     5941 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat 
     6001 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 
     6061 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 
     6121 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc 
     6181 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 
     6241 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 
     6301 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca 
     6361 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 
     6421 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 
     6481 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta 
     6541 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 
     6601 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 
     6661 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 
     6721 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 
     6781 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 
     6841 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 
     6901 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta 
     6961 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 
     7021 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 
     7081 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct 
     7141 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag 
     7201 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta 
     7261 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc 
     7321 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca 
     7381 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg 
     7441 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg 
     7501 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca 
     7561 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa 
     7621 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa 
     7681 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 
     7741 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 
     7801 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc 
     7861 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 
     7921 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 
     7981 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 
     8041 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 
     8101 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 
     8161 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 
     8221 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 
     8281 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 
     8341 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 
     8401 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 
     8461 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 
     8521 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 
     8581 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 
     8641 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 
     8701 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 
     8761 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 
     8821 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 
     8881 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt 
     8941 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc 
     9001 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc 
     9061 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct 
     9121 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc 
     9181 tgc 
//
