LOCUS       pLV-hU6-EF1a-RFP-Bsd     8982 bp    DNA     circular     26-SEP-2010
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     CDS             6903..7763
                     /label=Amp(R)
     promoter        7964..8504
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5508..5742
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6317..6772)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5814..5944
                     /label=SV40 pA
     misc_feature    5945..6158
                     /label=SV40 ori
     promoter        2278..2673
                     /label=human EF1a promoter
     intron          2706..3648
                     /label=intron
     promoter        complement(1872..2135)
                     /label=human U6
     CDS             4421..4819
                     /label=Bsd
     CDS             3695..4402
                     /label=RFP
     stem_loop       complement(1824..1871)
                     /label=shRNA
BASE COUNT     2201 a      2232 c      2353 g      2158 t        38 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnttggatcc aannnnnnnn 
     1861 nnnnnnnnnn ntttttttcg tcctttccac aagatatata aagccaagaa atcgaaatac 
     1921 tttcaagtta cggtaagcat atgatagtcc attttaaaac ataattttaa aactgcaaac 
     1981 tacccaagaa attattactt tctacgtcac gtattttgta ctaatatctt tgtgtttaca 
     2041 gtcaaattaa ttctaattat ctctctaaca gccttgtatc gtatatgcaa atatgaagga 
     2101 atcatgggaa ataggccctc ttcctgcccg accttgaatt cggccggatc caacttttaa 
     2161 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac 
     2221 agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgatgcc 
     2281 tccccgtcac cacccccccc aacccgcccc gaccggagct gagagtaatt catacaaaag 
     2341 gactcgcccc tgccttgggg aatcccaggg accgtcgtta aactcccact aacgtagaac 
     2401 ccagagatcg ctgcgttccc gccccctcac ccgcccgctc tcgtcatcac tgaggtggag 
     2461 aagagcatgc gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc 
     2521 ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg 
     2581 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac 
     2641 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa 
     2701 cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt 
     2761 gcgtgccttg aattacttcc acgcccctgg ctgcagtacg tgattcttga tcccgagctt 
     2821 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt 
     2881 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt 
     2941 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgatatcct 
     3001 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt 
     3061 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg 
     3121 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc 
     3181 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg 
     3241 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca 
     3301 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa 
     3361 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg 
     3421 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg 
     3481 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca 
     3541 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc 
     3601 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgaaaa 
     3661 ctacccctct agagtcgagc taccggtcgc caccatggtg agcaagggcg aggaggataa 
     3721 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg 
     3781 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 
     3841 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca 
     3901 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa 
     3961 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt 
     4021 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct 
     4081 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga 
     4141 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag 
     4201 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa 
     4261 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca 
     4321 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg 
     4381 cggcatggac gagctgtaca aggacccacc ggtcgccacc atggccaagc ctttgtctca 
     4441 agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga 
     4501 agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa 
     4561 tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc 
     4621 tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt 
     4681 gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat 
     4741 agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg 
     4801 ttatgtgtgg gagggctaag cggccgcgtc gacaatcaac ctctggatta caaaatttgt 
     4861 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 
     4921 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 
     4981 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 
     5041 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 
     5101 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 
     5161 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 
     5221 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc 
     5281 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 
     5341 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc 
     5401 tccctttggg ccgcctcccc gcctggaatt cgagctcggt acctttaaga ccaatgactt 
     5461 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa 
     5521 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc 
     5581 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 
     5641 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 
     5701 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc 
     5761 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt 
     5821 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 
     5881 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 
     5941 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 
     6001 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 
     6061 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta 
     6121 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt 
     6181 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc 
     6241 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc 
     6301 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 
     6361 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 
     6421 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 
     6481 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 
     6541 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 
     6601 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 
     6661 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 
     6721 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca 
     6781 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 
     6841 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 
     6901 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 
     6961 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 
     7021 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 
     7081 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 
     7141 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 
     7201 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 
     7261 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 
     7321 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 
     7381 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 
     7441 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 
     7501 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 
     7561 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 
     7621 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 
     7681 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 
     7741 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 
     7801 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 
     7861 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 
     7921 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 
     7981 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 
     8041 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt 
     8101 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 
     8161 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 
     8221 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 
     8281 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 
     8341 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 
     8401 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 
     8461 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 
     8521 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 
     8581 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 
     8641 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 
     8701 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 
     8761 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt 
     8821 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg 
     8881 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc 
     8941 gcgcaattaa ccctcactaa agggaacaaa agctggagct gc 
//
