LOCUS       pLV-hU6-EF1a-GFP-Bsd    8988 bp    DNA     circular     26-SEP-2010
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     CDS             6909..7769
                     /label=Amp(R)
     promoter        7970..8510
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5514..5748
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6323..6778)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5820..5950
                     /label=SV40 pA
     misc_feature    5951..6164
                     /label=SV40 ori
     promoter        2278..2673
                     /label=human EF1a promoter
     intron          2706..3648
                     /label=intron
     promoter        complement(1872..2135)
                     /label=human U6
     CDS             3692..4408
                     /label=GFP
     CDS             4427..4825
                     /label=Bsd
     stem_loop       complement(1824..1871)
                     /label=shRNA
BASE COUNT     2210 a      2247 c      2334 g      2159 t        38 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnttggatcc aannnnnnnn 
     1861 nnnnnnnnnn ntttttttcg tcctttccac aagatatata aagccaagaa atcgaaatac 
     1921 tttcaagtta cggtaagcat atgatagtcc attttaaaac ataattttaa aactgcaaac 
     1981 tacccaagaa attattactt tctacgtcac gtattttgta ctaatatctt tgtgtttaca 
     2041 gtcaaattaa ttctaattat ctctctaaca gccttgtatc gtatatgcaa atatgaagga 
     2101 atcatgggaa ataggccctc ttcctgcccg accttgaatt cggccggatc caacttttaa 
     2161 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac 
     2221 agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgatgcc 
     2281 tccccgtcac cacccccccc aacccgcccc gaccggagct gagagtaatt catacaaaag 
     2341 gactcgcccc tgccttgggg aatcccaggg accgtcgtta aactcccact aacgtagaac 
     2401 ccagagatcg ctgcgttccc gccccctcac ccgcccgctc tcgtcatcac tgaggtggag 
     2461 aagagcatgc gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc 
     2521 ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg 
     2581 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac 
     2641 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa 
     2701 cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt 
     2761 gcgtgccttg aattacttcc acgcccctgg ctgcagtacg tgattcttga tcccgagctt 
     2821 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt 
     2881 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt 
     2941 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgatatcct 
     3001 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt 
     3061 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg 
     3121 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc 
     3181 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg 
     3241 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca 
     3301 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa 
     3361 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg 
     3421 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg 
     3481 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca 
     3541 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc 
     3601 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgaaaa 
     3661 ctacccctct agagatccac cggtcgccac catggtgagc aagggcgagg agctgttcac 
     3721 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt 
     3781 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac 
     3841 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca 
     3901 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc 
     3961 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg 
     4021 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga 
     4081 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa 
     4141 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca 
     4201 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg 
     4261 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa 
     4321 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat 
     4381 cactctcggc atggacgagc tgtacaagga cccaccggtc gccaccatgg ccaagccttt 
     4441 gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat 
     4501 ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg 
     4561 tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc 
     4621 tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg 
     4681 catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa 
     4741 agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc 
     4801 ctctggttat gtgtgggagg gctaagcggc cgcgtcgaca atcaacctct ggattacaaa 
     4861 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac 
     4921 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc 
     4981 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt 
     5041 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc 
     5101 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc 
     5161 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg 
     5221 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt 
     5281 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc 
     5341 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt 
     5401 cggatctccc tttgggccgc ctccccgcct ggaattcgag ctcggtacct ttaagaccaa 
     5461 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag 
     5521 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt 
     5581 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 
     5641 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 
     5701 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat 
     5761 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga 
     5821 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 
     5881 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 
     5941 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc 
     6001 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 
     6061 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 
     6121 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta 
     6181 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc 
     6241 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg 
     6301 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 
     6361 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 
     6421 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg 
     6481 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag 
     6541 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 
     6601 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 
     6661 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat 
     6721 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag 
     6781 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 
     6841 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 
     6901 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 
     6961 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 
     7021 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 
     7081 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 
     7141 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 
     7201 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 
     7261 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 
     7321 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 
     7381 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 
     7441 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 
     7501 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 
     7561 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 
     7621 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 
     7681 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 
     7741 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 
     7801 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 
     7861 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 
     7921 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 
     7981 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 
     8041 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc 
     8101 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 
     8161 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 
     8221 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 
     8281 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 
     8341 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 
     8401 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 
     8461 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 
     8521 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 
     8581 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 
     8641 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 
     8701 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 
     8761 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 
     8821 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 
     8881 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg 
     8941 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgc 
//
