LOCUS       pLV-mU6-EF1a-GFP-Puro    9240 bp    DNA     circular     22-SEP-2010
SOURCE      
COMMENT     
            This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     CDS             7161..8021
                     /label=Amp(R)
     promoter        8222..8762
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5766..6000
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6575..7030)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6072..6202
                     /label=SV40 pA
     misc_feature    6203..6416
                     /label=SV40 ori
     promoter        2329..2724
                     /label=human EF1a promoter
     intron          2757..3699
                     /label=intron
     promoter        complement(1872..2186)
                     /label=mouse U6
     stem_loop       complement(1824..1871)
                     /label=shRNA
     CDS             3743..5077
                     /label=GFP-Puro
BASE COUNT     2210 a      2380 c      2459 g      2153 t        38 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnttggatcc aannnnnnnn 
     1861 nnnnnnnnnn ntttttaggc ttttctccaa gggatattta tagtctcaaa acacacaatt 
     1921 actttacagt tagggtgagt ttccttttgt gctgtttttt aaaataataa tttagtattt 
     1981 gtatctctta tagaaatcca agcctatcat gtaaaatgta gctagtatta aaaagaacag 
     2041 attatctgtc ttttatcgca cattaagcct ctatagttac taggaaatat tatatgcaaa 
     2101 ttaaccgggg caggggagta gccgagcttc tcccacaagt ctgtgcgagg gggccggcgc 
     2161 gggcctagag atggcggcgt cggatcgaat tcggccggat ccaactttta aaagaaaagg 
     2221 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca 
     2281 aactaaagaa ttacaaaaac aaattacaaa attcaaaatt ttatcgatgc ctccccgtca 
     2341 ccaccccccc caacccgccc cgaccggagc tgagagtaat tcatacaaaa ggactcgccc 
     2401 ctgccttggg gaatcccagg gaccgtcgtt aaactcccac taacgtagaa cccagagatc 
     2461 gctgcgttcc cgccccctca cccgcccgct ctcgtcatca ctgaggtgga gaagagcatg 
     2521 cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt 
     2581 tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg 
     2641 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa 
     2701 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa 
     2761 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt 
     2821 gaattacttc cacgcccctg gctgcagtac gtgattcttg atcccgagct tcgggttgga 
     2881 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt 
     2941 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt 
     3001 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgatatcc tgcgacgctt 
     3061 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt 
     3121 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg 
     3181 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct 
     3241 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg 
     3301 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca 
     3361 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg 
     3421 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg 
     3481 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt 
     3541 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac 
     3601 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag 
     3661 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgaaa actacccctc 
     3721 tagagatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt 
     3781 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 
     3841 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 
     3901 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag 
     3961 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta 
     4021 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 
     4081 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 
     4141 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 
     4201 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 
     4261 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 
     4321 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa 
     4381 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 
     4441 catggacgag ctgtacaagg acccaccggt cgccaccatg accgagtaca agcccacggt 
     4501 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc 
     4561 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga 
     4621 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga 
     4681 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt 
     4741 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca 
     4801 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt 
     4861 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt 
     4921 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct 
     4981 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc 
     5041 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagcg gccgcgtcga caatcaacct 
     5101 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 
     5161 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 
     5221 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 
     5281 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 
     5341 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 
     5401 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 
     5461 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt 
     5521 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 
     5581 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc 
     5641 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattcg agctcggtac 
     5701 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg 
     5761 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg 
     5821 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 
     5881 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 
     5941 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 
     6001 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag 
     6061 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 
     6121 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 
     6181 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc 
     6241 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 
     6301 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 
     6361 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg 
     6421 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt 
     6481 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct 
     6541 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc 
     6601 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 
     6661 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 
     6721 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 
     6781 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 
     6841 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 
     6901 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 
     6961 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg 
     7021 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 
     7081 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 
     7141 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 
     7201 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 
     7261 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 
     7321 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 
     7381 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 
     7441 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 
     7501 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 
     7561 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 
     7621 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 
     7681 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 
     7741 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 
     7801 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 
     7861 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 
     7921 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 
     7981 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 
     8041 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 
     8101 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 
     8161 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 
     8221 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 
     8281 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 
     8341 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 
     8401 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 
     8461 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 
     8521 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 
     8581 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 
     8641 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 
     8701 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 
     8761 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 
     8821 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 
     8881 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 
     8941 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 
     9001 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 
     9061 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 
     9121 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 
     9181 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc 
//
