LOCUS       pLV-H1-EF1a-GFP-Puro    9148 bp    DNA     circular     22-SEP-2010
SOURCE      
COMMENT     
            This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     CDS             7069..7929
                     /label=Amp(R)
     promoter        8130..8670
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5674..5908
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6483..6938)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5980..6110
                     /label=SV40 pA
     misc_feature    6111..6324
                     /label=SV40 ori
     misc_binding    418..435
                     /label=PBS
     promoter        2237..2632
                     /label=human EF1a promoter
     intron          2665..3607
                     /label=intron
     promoter        complement(1872..2100)
                     /label=H1
     CDS             3651..4985
                     /label=GFP-Puro
     stem_loop       complement(1824..1871)
                     /label=shRNA
BASE COUNT     2170 a      2389 c      2444 g      2107 t        38 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnttggatcc aannnnnnnn 
     1861 nnnnnnnnnn ntttttagag tggtctcata cagaacttat aagattccca aatccaaaga 
     1921 catttcacgt ttatggtgat ttcccagaac acatagcgac atgcaaatat tgcagggcgc 
     1981 cactcccctg tccctcacag ccatcttcct gccagggcgc acgcgcgctg ggtgttcccg 
     2041 cctagtgaca ctgggcccgc gattccttgg agcgggttga tgacgtcagc gttcgaattc 
     2101 ggccggatcc aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa 
     2161 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat 
     2221 tcaaaatttt atcgatgcct ccccgtcacc acccccccca acccgccccg accggagctg 
     2281 agagtaattc atacaaaagg actcgcccct gccttgggga atcccaggga ccgtcgttaa 
     2341 actcccacta acgtagaacc cagagatcgc tgcgttcccg ccccctcacc cgcccgctct 
     2401 cgtcatcact gaggtggaga agagcatgcg tgaggctccg gtgcccgtca gtgggcagag 
     2461 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc 
     2521 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt 
     2581 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc 
     2641 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc 
     2701 tttacgggtt atggcccttg cgtgccttga attacttcca cgcccctggc tgcagtacgt 
     2761 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta 
     2821 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt 
     2881 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta 
     2941 aaatttttga tgatatcctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg 
     3001 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc 
     3061 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg 
     3121 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc 
     3181 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc 
     3241 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc 
     3301 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga 
     3361 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac 
     3421 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt 
     3481 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt 
     3541 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc 
     3601 atttcaggtg tcgtgaaaac tacccctcta gagatccacc ggtcgccacc atggtgagca 
     3661 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 
     3721 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga 
     3781 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 
     3841 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact 
     3901 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg 
     3961 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 
     4021 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 
     4081 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg 
     4141 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc 
     4201 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca 
     4261 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt 
     4321 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggac ccaccggtcg 
     4381 ccaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg 
     4441 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc 
     4501 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 
     4561 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 
     4621 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 
     4681 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 
     4741 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 
     4801 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 
     4861 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 
     4921 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg 
     4981 cctgagcggc cgcgtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg 
     5041 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 
     5101 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 
     5161 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 
     5221 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac 
     5281 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 
     5341 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac 
     5401 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg 
     5461 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 
     5521 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc 
     5581 ctccccgcct ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta 
     5641 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga 
     5701 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg 
     5761 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 
     5821 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 
     5881 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat 
     5941 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat 
     6001 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 
     6061 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta 
     6121 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 
     6181 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 
     6241 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta 
     6301 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa 
     6361 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa 
     6421 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg 
     6481 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 
     6541 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 
     6601 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 
     6661 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 
     6721 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 
     6781 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 
     6841 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 
     6901 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat 
     6961 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 
     7021 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 
     7081 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 
     7141 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 
     7201 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 
     7261 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 
     7321 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 
     7381 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 
     7441 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 
     7501 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 
     7561 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 
     7621 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 
     7681 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 
     7741 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 
     7801 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 
     7861 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 
     7921 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 
     7981 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 
     8041 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 
     8101 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 
     8161 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 
     8221 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc 
     8281 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 
     8341 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 
     8401 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 
     8461 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 
     8521 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 
     8581 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 
     8641 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 
     8701 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 
     8761 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 
     8821 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata 
     8881 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 
     8941 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 
     9001 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga 
     9061 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct 
     9121 cactaaaggg aacaaaagct ggagctgc 
//
