LOCUS       pLV-mU6-EF1a-RFP-Bsd     9033 bp    DNA     circular     20-DEC-2009
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     CDS             6954..7814
                     /label=Amp(R)
     promoter        8015..8555
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5559..5793
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6368..6823)
                     /label=f1 ori
     LTR             236..416
                     /label=5' LTR
     polyA_signal    5865..5995
                     /label=SV40 pA
     misc_feature    5996..6209
                     /label=SV40 ori
     promoter        2329..2724
                     /label=human EF1a promoter
     intron          2757..3699
                     /label=intron
     promoter        complement(1872..2186)
                     /label=mouse U6 promoter
     CDS             3746..4870
                     /label=RFP-Bsd
     stem_loop       1824..1871
                     /label=shRNA
BASE COUNT     2203 a      2236 c      2388 g      2168 t        38 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnttggatcc aannnnnnnn 
     1861 nnnnnnnnnn ntttttaggc ttttctccaa gggatattta tagtctcaaa acacacaatt 
     1921 actttacagt tagggtgagt ttccttttgt gctgtttttt aaaataataa tttagtattt 
     1981 gtatctctta tagaaatcca agcctatcat gtaaaatgta gctagtatta aaaagaacag 
     2041 attatctgtc ttttatcgca cattaagcct ctatagttac taggaaatat tatatgcaaa 
     2101 ttaaccgggg caggggagta gccgagcttc tcccacaagt ctgtgcgagg gggccggcgc 
     2161 gggcctagag atggcggcgt cggatcgaat tcggccggat ccaactttta aaagaaaagg 
     2221 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca 
     2281 aactaaagaa ttacaaaaac aaattacaaa attcaaaatt ttatcgatgc ctccccgtca 
     2341 ccaccccccc caacccgccc cgaccggagc tgagagtaat tcatacaaaa ggactcgccc 
     2401 ctgccttggg gaatcccagg gaccgtcgtt aaactcccac taacgtagaa cccagagatc 
     2461 gctgcgttcc cgccccctca cccgcccgct ctcgtcatca ctgaggtgga gaagagcatg 
     2521 cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt 
     2581 tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg 
     2641 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa 
     2701 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa 
     2761 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt 
     2821 gaattacttc cacgcccctg gctgcagtac gtgattcttg atcccgagct tcgggttgga 
     2881 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt 
     2941 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt 
     3001 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgatatcc tgcgacgctt 
     3061 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt 
     3121 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg 
     3181 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct 
     3241 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg 
     3301 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca 
     3361 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg 
     3421 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg 
     3481 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt 
     3541 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac 
     3601 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag 
     3661 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgaaa actacccctc 
     3721 tagagtcgag ctaccggtcg ccaccatggt gagcaagggc gaggaggata acatggccat 
     3781 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt 
     3841 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa 
     3901 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta 
     3961 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt 
     4021 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt 
     4081 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac 
     4141 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc 
     4201 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct 
     4261 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt 
     4321 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga 
     4381 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga 
     4441 cgagctgtac aaggacccac cggtcgccac catggccaag cctttgtctc aagaagaatc 
     4501 caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag 
     4561 cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca 
     4621 ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc 
     4681 tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg 
     4741 cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga 
     4801 cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg 
     4861 ggagggctaa gcggccgcgt cgacaatcaa cctctggatt acaaaatttg tgaaagattg 
     4921 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 
     4981 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 
     5041 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 
     5101 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 
     5161 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 
     5221 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag 
     5281 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 
     5341 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 
     5401 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg 
     5461 gccgcctccc cgcctggaat tcgagctcgg tacctttaag accaatgact tacaaggcag 
     5521 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc 
     5581 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag 
     5641 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt 
     5701 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca 
     5761 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc 
     5821 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag 
     5881 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 
     5941 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct 
     6001 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 
     6061 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 
     6121 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg 
     6181 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg 
     6241 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg 
     6301 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc 
     6361 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc 
     6421 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt 
     6481 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc 
     6541 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt 
     6601 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt 
     6661 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt 
     6721 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa 
     6781 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg 
     6841 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 
     6901 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 
     6961 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 
     7021 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 
     7081 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 
     7141 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg 
     7201 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 
     7261 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 
     7321 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 
     7381 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 
     7441 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 
     7501 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 
     7561 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 
     7621 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 
     7681 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 
     7741 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 
     7801 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 
     7861 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 
     7921 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 
     7981 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 
     8041 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 
     8101 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc 
     8161 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 
     8221 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 
     8281 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 
     8341 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 
     8401 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 
     8461 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 
     8521 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 
     8581 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 
     8641 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 
     8701 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc 
     8761 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 
     8821 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 
     8881 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 
     8941 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta 
     9001 accctcacta aagggaacaa aagctggagc tgc 
//
