LOCUS       pLV-H1-EF1a-RFP-Bsd    8941 bp    DNA     circular     20-DEC-2009
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     CDS             6862..7722
                     /label=Amp(R)
     promoter        7923..8463
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5467..5701
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6276..6731)
                     /label=f1 ori
     LTR             236..416
                     /label=5' LTR
     polyA_signal    5773..5903
                     /label=SV40 pA
     misc_feature    5904..6117
                     /label=SV40 ori
     promoter        2237..2632
                     /label=human EF1a promoter
     intron          2665..3607
                     /label=intron
     promoter        complement(1872..2100)
                     /label=H1 promoter
     CDS             3654..4778
                     /label=RFP-Bsd
     stem_loop       1824..1871
                     /label=shRNA
BASE COUNT     2163 a      2245 c      2373 g      2122 t        38 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnttggatcc aannnnnnnn 
     1861 nnnnnnnnnn ntttttagag tggtctcata cagaacttat aagattccca aatccaaaga 
     1921 catttcacgt ttatggtgat ttcccagaac acatagcgac atgcaaatat tgcagggcgc 
     1981 cactcccctg tccctcacag ccatcttcct gccagggcgc acgcgcgctg ggtgttcccg 
     2041 cctagtgaca ctgggcccgc gattccttgg agcgggttga tgacgtcagc gttcgaattc 
     2101 ggccggatcc aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa 
     2161 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat 
     2221 tcaaaatttt atcgatgcct ccccgtcacc acccccccca acccgccccg accggagctg 
     2281 agagtaattc atacaaaagg actcgcccct gccttgggga atcccaggga ccgtcgttaa 
     2341 actcccacta acgtagaacc cagagatcgc tgcgttcccg ccccctcacc cgcccgctct 
     2401 cgtcatcact gaggtggaga agagcatgcg tgaggctccg gtgcccgtca gtgggcagag 
     2461 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc 
     2521 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt 
     2581 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc 
     2641 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc 
     2701 tttacgggtt atggcccttg cgtgccttga attacttcca cgcccctggc tgcagtacgt 
     2761 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta 
     2821 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt 
     2881 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta 
     2941 aaatttttga tgatatcctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg 
     3001 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc 
     3061 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg 
     3121 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc 
     3181 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc 
     3241 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc 
     3301 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga 
     3361 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac 
     3421 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt 
     3481 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt 
     3541 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc 
     3601 atttcaggtg tcgtgaaaac tacccctcta gagtcgagct accggtcgcc accatggtga 
     3661 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca 
     3721 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct 
     3781 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct 
     3841 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg 
     3901 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga 
     3961 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt 
     4021 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga 
     4081 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga 
     4141 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca 
     4201 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca 
     4261 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg 
     4321 agggccgcca ctccaccggc ggcatggacg agctgtacaa ggacccaccg gtcgccacca 
     4381 tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca 
     4441 acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc 
     4501 gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg 
     4561 tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa 
     4621 atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc 
     4681 atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc 
     4741 gtgaattgct gccctctggt tatgtgtggg agggctaagc ggccgcgtcg acaatcaacc 
     4801 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 
     4861 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 
     4921 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 
     4981 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 
     5041 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 
     5101 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 
     5161 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt 
     5221 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 
     5281 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 
     5341 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc gagctcggta 
     5401 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag 
     5461 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact 
     5521 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 
     5581 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 
     5641 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 
     5701 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca 
     5761 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 
     5821 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 
     5881 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc 
     5941 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 
     6001 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 
     6061 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact 
     6121 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct 
     6181 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc 
     6241 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag 
     6301 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc 
     6361 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc 
     6421 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa 
     6481 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg 
     6541 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac 
     6601 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta 
     6661 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac 
     6721 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt 
     6781 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa 
     6841 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt 
     6901 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat 
     6961 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag 
     7021 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg 
     7081 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata 
     7141 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat 
     7201 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc 
     7261 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg 
     7321 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac 
     7381 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact 
     7441 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa 
     7501 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct 
     7561 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc 
     7621 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga 
     7681 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac 
     7741 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 
     7801 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 
     7861 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 
     7921 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 
     7981 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt 
     8041 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 
     8101 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 
     8161 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 
     8221 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 
     8281 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 
     8341 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 
     8401 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 
     8461 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 
     8521 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt 
     8581 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag 
     8641 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg 
     8701 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc 
     8761 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt 
     8821 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat 
     8881 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg 
     8941 c 
//
