LOCUS       pLV-mU6-EF1a-GFP-Bsd    9039 bp    DNA     circular     20-DEC-2009
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     CDS             6960..7820
                     /label=Amp(R)
     promoter        8021..8561
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5565..5799
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6374..6829)
                     /label=f1 ori
     LTR             236..416
                     /label=5' LTR
     polyA_signal    5871..6001
                     /label=SV40 pA
     misc_feature    6002..6215
                     /label=SV40 ori
     promoter        2329..2724
                     /label=human EF1a promoter
     intron          2757..3699
                     /label=intron
     promoter        complement(1872..2186)
                     /label=mouse U6 promoter
     CDS             3743..4876
                     /label=GFP-Bsd
     stem_loop       1824..1871
                     /label=shRNA
BASE COUNT     2212 a      2251 c      2369 g      2169 t        38 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnttggatcc aannnnnnnn 
     1861 nnnnnnnnnn ntttttaggc ttttctccaa gggatattta tagtctcaaa acacacaatt 
     1921 actttacagt tagggtgagt ttccttttgt gctgtttttt aaaataataa tttagtattt 
     1981 gtatctctta tagaaatcca agcctatcat gtaaaatgta gctagtatta aaaagaacag 
     2041 attatctgtc ttttatcgca cattaagcct ctatagttac taggaaatat tatatgcaaa 
     2101 ttaaccgggg caggggagta gccgagcttc tcccacaagt ctgtgcgagg gggccggcgc 
     2161 gggcctagag atggcggcgt cggatcgaat tcggccggat ccaactttta aaagaaaagg 
     2221 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca 
     2281 aactaaagaa ttacaaaaac aaattacaaa attcaaaatt ttatcgatgc ctccccgtca 
     2341 ccaccccccc caacccgccc cgaccggagc tgagagtaat tcatacaaaa ggactcgccc 
     2401 ctgccttggg gaatcccagg gaccgtcgtt aaactcccac taacgtagaa cccagagatc 
     2461 gctgcgttcc cgccccctca cccgcccgct ctcgtcatca ctgaggtgga gaagagcatg 
     2521 cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt 
     2581 tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg 
     2641 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa 
     2701 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa 
     2761 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt 
     2821 gaattacttc cacgcccctg gctgcagtac gtgattcttg atcccgagct tcgggttgga 
     2881 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt 
     2941 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt 
     3001 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgatatcc tgcgacgctt 
     3061 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt 
     3121 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg 
     3181 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct 
     3241 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg 
     3301 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca 
     3361 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg 
     3421 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg 
     3481 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt 
     3541 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac 
     3601 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag 
     3661 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgaaa actacccctc 
     3721 tagagatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt 
     3781 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 
     3841 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 
     3901 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag 
     3961 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta 
     4021 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 
     4081 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 
     4141 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 
     4201 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 
     4261 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 
     4321 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa 
     4381 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 
     4441 catggacgag ctgtacaagg acccaccggt cgccaccatg gccaagcctt tgtctcaaga 
     4501 agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga 
     4561 ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt 
     4621 atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc 
     4681 ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag 
     4741 cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt 
     4801 gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta 
     4861 tgtgtgggag ggctaagcgg ccgcgtcgac aatcaacctc tggattacaa aatttgtgaa 
     4921 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 
     4981 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 
     5041 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 
     5101 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 
     5161 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 
     5221 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 
     5281 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg 
     5341 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 
     5401 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc 
     5461 ctttgggccg cctccccgcc tggaattcga gctcggtacc tttaagacca atgacttaca 
     5521 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc 
     5581 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga 
     5641 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct 
     5701 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat 
     5761 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta 
     5821 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta 
     5881 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 
     5941 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 
     6001 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc 
     6061 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 
     6121 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc 
     6181 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt 
     6241 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc 
     6301 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg 
     6361 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 
     6421 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 
     6481 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta 
     6541 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 
     6601 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg 
     6661 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat 
     6721 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt 
     6781 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt 
     6841 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 
     6901 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 
     6961 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 
     7021 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 
     7081 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 
     7141 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 
     7201 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 
     7261 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 
     7321 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 
     7381 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 
     7441 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 
     7501 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 
     7561 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 
     7621 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 
     7681 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 
     7741 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 
     7801 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt 
     7861 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 
     7921 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 
     7981 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 
     8041 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 
     8101 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag 
     8161 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 
     8221 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 
     8281 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 
     8341 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 
     8401 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 
     8461 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 
     8521 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 
     8581 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 
     8641 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg 
     8701 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag 
     8761 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 
     8821 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 
     8881 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 
     8941 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg 
     9001 caattaaccc tcactaaagg gaacaaaagc tggagctgc 
//
