LOCUS       pLV-H1-EF1a-GFP-Bsd    8947 bp    DNA     circular     20-DEC-2009
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     CDS             6868..7728
                     /label=Amp(R)
     promoter        7929..8469
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5473..5707
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6282..6737)
                     /label=f1 ori
     LTR             236..416
                     /label=5' LTR
     polyA_signal    5779..5909
                     /label=SV40 pA
     misc_feature    5910..6123
                     /label=SV40 ori
     promoter        2237..2632
                     /label=human EF1a promoter
     intron          2665..3607
                     /label=intron
     promoter        complement(1872..2100)
                     /label=H1 promoter
     CDS             3651..4784
                     /label=GFP-Bsd
     stem_loop       1824..1871
                     /label=shRNA
BASE COUNT     2172 a      2260 c      2354 g      2123 t        38 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnttggatcc aannnnnnnn 
     1861 nnnnnnnnnn ntttttagag tggtctcata cagaacttat aagattccca aatccaaaga 
     1921 catttcacgt ttatggtgat ttcccagaac acatagcgac atgcaaatat tgcagggcgc 
     1981 cactcccctg tccctcacag ccatcttcct gccagggcgc acgcgcgctg ggtgttcccg 
     2041 cctagtgaca ctgggcccgc gattccttgg agcgggttga tgacgtcagc gttcgaattc 
     2101 ggccggatcc aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa 
     2161 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat 
     2221 tcaaaatttt atcgatgcct ccccgtcacc acccccccca acccgccccg accggagctg 
     2281 agagtaattc atacaaaagg actcgcccct gccttgggga atcccaggga ccgtcgttaa 
     2341 actcccacta acgtagaacc cagagatcgc tgcgttcccg ccccctcacc cgcccgctct 
     2401 cgtcatcact gaggtggaga agagcatgcg tgaggctccg gtgcccgtca gtgggcagag 
     2461 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc 
     2521 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt 
     2581 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc 
     2641 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc 
     2701 tttacgggtt atggcccttg cgtgccttga attacttcca cgcccctggc tgcagtacgt 
     2761 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta 
     2821 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt 
     2881 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta 
     2941 aaatttttga tgatatcctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg 
     3001 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc 
     3061 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg 
     3121 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc 
     3181 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc 
     3241 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc 
     3301 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga 
     3361 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac 
     3421 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt 
     3481 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt 
     3541 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc 
     3601 atttcaggtg tcgtgaaaac tacccctcta gagatccacc ggtcgccacc atggtgagca 
     3661 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 
     3721 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga 
     3781 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 
     3841 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact 
     3901 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg 
     3961 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 
     4021 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 
     4081 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg 
     4141 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc 
     4201 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca 
     4261 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt 
     4321 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggac ccaccggtcg 
     4381 ccaccatggc caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta 
     4441 caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg 
     4501 acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac 
     4561 tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga 
     4621 tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg 
     4681 atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg 
     4741 ggattcgtga attgctgccc tctggttatg tgtgggaggg ctaagcggcc gcgtcgacaa 
     4801 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 
     4861 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 
     4921 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 
     4981 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 
     5041 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 
     5101 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 
     5161 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc 
     5221 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 
     5281 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 
     5341 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcgagc 
     5401 tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa 
     5461 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct 
     5521 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 
     5581 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 
     5641 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 
     5701 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga 
     5761 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat 
     5821 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 
     5881 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca 
     5941 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 
     6001 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 
     6061 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg 
     6121 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa 
     6181 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga 
     6241 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc 
     6301 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 
     6361 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 
     6421 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 
     6481 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 
     6541 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 
     6601 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 
     6661 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 
     6721 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 
     6781 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 
     6841 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 
     6901 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 
     6961 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 
     7021 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 
     7081 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 
     7141 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 
     7201 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 
     7261 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 
     7321 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 
     7381 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 
     7441 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 
     7501 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 
     7561 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 
     7621 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 
     7681 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 
     7741 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 
     7801 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 
     7861 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 
     7921 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 
     7981 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 
     8041 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 
     8101 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 
     8161 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 
     8221 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 
     8281 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 
     8341 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 
     8401 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 
     8461 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 
     8521 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 
     8581 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 
     8641 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 
     8701 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 
     8761 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt 
     8821 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac 
     8881 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg 
     8941 gagctgc 
//
