LOCUS       pLV-mU6-EF1a-Bsd        8308 bp    DNA     circular     31-JUL-2008

FEATURES             Location/Qualifiers
     CDS             6229..7089
                     /gene=Amp(R)
     promoter        7290..7830
                     /gene=pUC ori
     promoter        7..235
                     /gene=RSV
     LTR             4834..5068
                     /gene=3'LTR
     intron          526..1690
                     /gene=Intron
     rep_origin      complement(5643..6098)
                     /gene=f1 ori
     LTR             236..416
                     /gene=5'LTR
     polyA_signal    5140..5270
                     /gene=SV40 pA
     misc_feature    5271..5484
                     /gene=SV40 ori
     promoter        2333..2728
                     /gene=human EF1a promoter
     intron          2761..3703
                     /gene=intron
     promoter        complement(1876..2190)
                     /gene=mouse U6 promoter
     stem_loop       1824..1875
                     /gene=shRNA
     CDS             3747..4145
                     /gene=Bsd(R)
                     
BASE COUNT     2037 a      2001 c      2162 g      2066 t        42 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 
     1861 nnnnnnnnnn nnnnnttttt aggcttttct ccaagggata tttatagtct caaaacacac 
     1921 aattacttta cagttagggt gagtttcctt ttgtgctgtt ttttaaaata ataatttagt 
     1981 atttgtatct cttatagaaa tccaagccta tcatgtaaaa tgtagctagt attaaaaaga 
     2041 acagattatc tgtcttttat cgcacattaa gcctctatag ttactaggaa atattatatg 
     2101 caaattaacc ggggcagggg agtagccgag cttctcccac aagtctgtgc gagggggccg 
     2161 gcgcgggcct agagatggcg gcgtcggatc gaattcggcc ggatccaact tttaaaagaa 
     2221 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca 
     2281 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg atgcctcccc 
     2341 gtcaccaccc cccccaaccc gccccgaccg gagctgagag taattcatac aaaaggactc 
     2401 gcccctgcct tggggaatcc cagggaccgt cgttaaactc ccactaacgt agaacccaga 
     2461 gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc atcactgagg tggagaagag 
     2521 catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 
     2581 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac 
     2641 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 
     2701 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 
     2761 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg 
     2821 ccttgaatta cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt 
     2881 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg 
     2941 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc 
     3001 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgat atcctgcgac 
     3061 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc 
     3121 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag 
     3181 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc 
     3241 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc 
     3301 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag 
     3361 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa 
     3421 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc 
     3481 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg 
     3541 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg 
     3601 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct 
     3661 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gaaaactacc 
     3721 cctctagaga tccaccggtc gccaccatgg ccaagccttt gtctcaagaa gaatccaccc 
     3781 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg 
     3841 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta 
     3901 ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca 
     3961 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac 
     4021 ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg 
     4081 atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg 
     4141 gctaagcggc cgcgtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg 
     4201 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 
     4261 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 
     4321 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 
     4381 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac 
     4441 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 
     4501 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac 
     4561 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg 
     4621 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 
     4681 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc 
     4741 ctccccgcct ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta 
     4801 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga 
     4861 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg 
     4921 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 
     4981 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 
     5041 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat 
     5101 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat 
     5161 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 
     5221 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta 
     5281 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 
     5341 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 
     5401 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta 
     5461 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa 
     5521 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa 
     5581 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg 
     5641 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 
     5701 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 
     5761 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 
     5821 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 
     5881 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 
     5941 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 
     6001 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 
     6061 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat 
     6121 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 
     6181 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 
     6241 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 
     6301 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 
     6361 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 
     6421 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 
     6481 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 
     6541 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 
     6601 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 
     6661 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 
     6721 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 
     6781 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 
     6841 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 
     6901 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 
     6961 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 
     7021 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 
     7081 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 
     7141 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 
     7201 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 
     7261 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 
     7321 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 
     7381 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc 
     7441 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 
     7501 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 
     7561 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 
     7621 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 
     7681 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 
     7741 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 
     7801 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 
     7861 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 
     7921 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 
     7981 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata 
     8041 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 
     8101 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 
     8161 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga 
     8221 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct 
     8281 cactaaaggg aacaaaagct ggagctgc 
//
