LOCUS       pLV-hU6-EF1a-Bsd        8257 bp    DNA     circular     31-JUL-2008

FEATURES             Location/Qualifiers
     CDS             6178..7038
                     /gene=Amp(R)
     promoter        7239..7779
                     /gene=pUC ori
     promoter        7..235
                     /gene=RSV
     LTR             4783..5017
                     /gene=3'LTR
     intron          526..1690
                     /gene=Intron
     rep_origin      complement(5592..6047)
                     /gene=f1 ori
     LTR             236..416
                     /gene=5'LTR
     polyA_signal    5089..5219
                     /gene=SV40 pA
     misc_feature    5220..5433
                     /gene=SV40 ori
     promoter        2282..2677
                     /gene=human EF1a promoter
     intron          2710..3652
                     /gene=intron
     stem_loop       1824..1875
                     /gene=shRNA
     promoter        complement(1876..2139)
                     /gene=human U6 promoter
     CDS             3696..4094
                     /gene=Bsd(R)
                     
BASE COUNT     2035 a      1997 c      2127 g      2056 t        42 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 
     1861 nnnnnnnnnn nnnnnttttt ttcgtccttt ccacaagata tataaagcca agaaatcgaa 
     1921 atactttcaa gttacggtaa gcatatgata gtccatttta aaacataatt ttaaaactgc 
     1981 aaactaccca agaaattatt actttctacg tcacgtattt tgtactaata tctttgtgtt 
     2041 tacagtcaaa ttaattctaa ttatctctct aacagccttg tatcgtatat gcaaatatga 
     2101 aggaatcatg ggaaataggc cctcttcctg cccgaccttg aattcggccg gatccaactt 
     2161 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag 
     2221 caacagacat acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcga 
     2281 tgcctccccg tcaccacccc ccccaacccg ccccgaccgg agctgagagt aattcataca 
     2341 aaaggactcg cccctgcctt ggggaatccc agggaccgtc gttaaactcc cactaacgta 
     2401 gaacccagag atcgctgcgt tcccgccccc tcacccgccc gctctcgtca tcactgaggt 
     2461 ggagaagagc atgcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 
     2521 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc 
     2581 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 
     2641 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 
     2701 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc 
     2761 ccttgcgtgc cttgaattac ttccacgccc ctggctgcag tacgtgattc ttgatcccga 
     2821 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc 
     2881 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca 
     2941 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgata 
     3001 tcctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac 
     3061 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg 
     3121 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc 
     3181 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc 
     3241 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc 
     3301 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac 
     3361 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg 
     3421 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg 
     3481 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag 
     3541 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg 
     3601 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg 
     3661 aaaactaccc ctctagagat ccaccggtcg ccaccatggc caagcctttg tctcaagaag 
     3721 aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact 
     3781 acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat 
     3841 atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg 
     3901 cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc 
     3961 cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga 
     4021 aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg 
     4081 tgtgggaggg ctaagcggcc gcgtcgacaa tcaacctctg gattacaaaa tttgtgaaag 
     4141 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat 
     4201 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc 
     4261 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg 
     4321 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct 
     4381 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct 
     4441 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg 
     4501 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac 
     4561 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct 
     4621 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct 
     4681 ttgggccgcc tccccgcctg gaattcgagc tcggtacctt taagaccaat gacttacaag 
     4741 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac 
     4801 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc 
     4861 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 
     4921 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 
     4981 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt 
     5041 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt 
     5101 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 
     5161 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 
     5221 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 
     5281 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 
     5341 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa 
     5401 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga 
     5461 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag 
     5521 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa 
     5581 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 
     5641 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 
     5701 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 
     5761 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 
     5821 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 
     5881 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 
     5941 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 
     6001 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt 
     6061 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 
     6121 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 
     6181 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 
     6241 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 
     6301 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 
     6361 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 
     6421 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 
     6481 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 
     6541 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 
     6601 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 
     6661 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 
     6721 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 
     6781 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 
     6841 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 
     6901 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 
     6961 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 
     7021 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 
     7081 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 
     7141 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 
     7201 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 
     7261 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 
     7321 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc 
     7381 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 
     7441 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 
     7501 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 
     7561 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 
     7621 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 
     7681 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 
     7741 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 
     7801 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 
     7861 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 
     7921 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 
     7981 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 
     8041 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc 
     8101 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt 
     8161 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca 
     8221 attaaccctc actaaaggga acaaaagctg gagctgc 
//
