LOCUS       pLV-H1-EF1a-Bsd         8216 bp    DNA     circular     31-JUL-2008

FEATURES             Location/Qualifiers
     CDS             6137..6997
                     /gene=Amp(R)
     promoter        7198..7738
                     /gene=pUC ori
     promoter        7..235
                     /gene=RSV
     LTR             4742..4976
                     /gene=3'LTR
     intron          526..1690
                     /gene=Intron
     rep_origin      complement(5551..6006)
                     /gene=f1 ori
     LTR             236..416
                     /gene=5'LTR
     polyA_signal    5048..5178
                     /gene=SV40 pA
     misc_feature    5179..5392
                     /gene=SV40 ori
     promoter        2241..2636
                     /gene=human EF1a promoter
     intron          2669..3611
                     /gene=intron
     stem_loop       1824..1875
                     /gene=shRNA
     promoter        complement(1876..2104)
                     /gene=H1 promoter
     CDS             3655..4053
                     /gene=Bsd(R)
                     
BASE COUNT     1997 a      2010 c      2147 g      2020 t        42 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 
     1861 nnnnnnnnnn nnnnnttttt agagtggtct catacagaac ttataagatt cccaaatcca 
     1921 aagacatttc acgtttatgg tgatttccca gaacacatag cgacatgcaa atattgcagg 
     1981 gcgccactcc cctgtccctc acagccatct tcctgccagg gcgcacgcgc gctgggtgtt 
     2041 cccgcctagt gacactgggc ccgcgattcc ttggagcggg ttgatgacgt cagcgttcga 
     2101 attcggccgg atccaacttt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 
     2161 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 
     2221 aaattcaaaa ttttatcgat gcctccccgt caccaccccc cccaacccgc cccgaccgga 
     2281 gctgagagta attcatacaa aaggactcgc ccctgccttg gggaatccca gggaccgtcg 
     2341 ttaaactccc actaacgtag aacccagaga tcgctgcgtt cccgccccct cacccgcccg 
     2401 ctctcgtcat cactgaggtg gagaagagca tgcgtgaggc tccggtgccc gtcagtgggc 
     2461 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg 
     2521 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct 
     2581 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt 
     2641 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg 
     2701 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacgcccc tggctgcagt 
     2761 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg 
     2821 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc 
     2881 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca 
     2941 tttaaaattt ttgatgatat cctgcgacgc tttttttctg gcaagatagt cttgtaaatg 
     3001 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc 
     3061 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg 
     3121 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta 
     3181 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat 
     3241 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc 
     3301 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat 
     3361 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga 
     3421 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt 
     3481 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc 
     3541 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc 
     3601 ttccatttca ggtgtcgtga aaactacccc tctagagatc caccggtcgc caccatggcc 
     3661 aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc 
     3721 atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc 
     3781 ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg 
     3841 ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag 
     3901 aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct 
     3961 gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa 
     4021 ttgctgccct ctggttatgt gtgggagggc taagcggccg cgtcgacaat caacctctgg 
     4081 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat 
     4141 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt 
     4201 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca 
     4261 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg 
     4321 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg 
     4381 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca 
     4441 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca 
     4501 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc 
     4561 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc 
     4621 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattcgagct cggtaccttt 
     4681 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg 
     4741 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc 
     4801 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
     4861 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
     4921 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 
     4981 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 
     5041 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 
     5101 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 
     5161 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta 
     5221 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 
     5281 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 
     5341 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg 
     5401 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag 
     5461 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc 
     5521 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg 
     5581 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 
     5641 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 
     5701 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 
     5761 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 
     5821 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 
     5881 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt 
     5941 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta 
     6001 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 
     6061 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 
     6121 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 
     6181 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 
     6241 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 
     6301 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 
     6361 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 
     6421 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 
     6481 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 
     6541 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 
     6601 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 
     6661 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 
     6721 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 
     6781 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 
     6841 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 
     6901 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 
     6961 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 
     7021 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 
     7081 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 
     7141 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 
     7201 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 
     7261 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct 
     7321 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 
     7381 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 
     7441 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 
     7501 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 
     7561 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 
     7621 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 
     7681 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 
     7741 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 
     7801 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 
     7861 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 
     7921 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 
     7981 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc 
     8041 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc 
     8101 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca 
     8161 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgc 
//
