LOCUS       pLV-mU6-EF1a-puro       8509 bp    DNA     circular     23-JUN-2008
SOURCE      
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     CDS             6430..7290
                     /gene=Amp(R)
                     /label=Amp(R)
     promoter        7491..8031
                     /gene=pUC ori
                     /label=pUC ori
     promoter        7..235
                     /gene=RSV
                     /label=RSV
     LTR             5035..5269
                     /gene=3'LTR
                     /label=3'LTR
     intron          526..1690
                     /gene=Intron
                     /label=Intron
     rep_origin      complement(5844..6299)
                     /gene=f1 ori
                     /label=f1 ori
     LTR             236..416
                     /gene=5'LTR
                     /label=5'LTR
     polyA_signal    5341..5471
                     /gene=SV40 pA
                     /label=SV40 pA
     misc_feature    5472..5685
                     /gene=SV40 ori
                     /label=SV40 ori
     promoter        2333..2728
                     /gene=human EF1a promoter
                     /label=human EF1a promoter
     intron          2761..3703
                     /gene=intron
                     /label=intron
     promoter        complement(1876..2190)
                     /gene=mouse U6 promoter
                     /label=mouse U6 promoter
     stem_loop       1824..1875
                     /gene=shRNA
                     /label=shRNA
     CDS             3747..4346
                     /gene=Puro(R)
                     
                     /label=Puro(R)
BASE COUNT     2035 a      2130 c      2252 g      2050 t        42 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 
     1861 nnnnnnnnnn nnnnnttttt aggcttttct ccaagggata tttatagtct caaaacacac 
     1921 aattacttta cagttagggt gagtttcctt ttgtgctgtt ttttaaaata ataatttagt 
     1981 atttgtatct cttatagaaa tccaagccta tcatgtaaaa tgtagctagt attaaaaaga 
     2041 acagattatc tgtcttttat cgcacattaa gcctctatag ttactaggaa atattatatg 
     2101 caaattaacc ggggcagggg agtagccgag cttctcccac aagtctgtgc gagggggccg 
     2161 gcgcgggcct agagatggcg gcgtcggatc gaattcggcc ggatccaact tttaaaagaa 
     2221 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca 
     2281 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg atgcctcccc 
     2341 gtcaccaccc cccccaaccc gccccgaccg gagctgagag taattcatac aaaaggactc 
     2401 gcccctgcct tggggaatcc cagggaccgt cgttaaactc ccactaacgt agaacccaga 
     2461 gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc atcactgagg tggagaagag 
     2521 catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 
     2581 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac 
     2641 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 
     2701 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 
     2761 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg 
     2821 ccttgaatta cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt 
     2881 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg 
     2941 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc 
     3001 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgat atcctgcgac 
     3061 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc 
     3121 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag 
     3181 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc 
     3241 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc 
     3301 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag 
     3361 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa 
     3421 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc 
     3481 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg 
     3541 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg 
     3601 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct 
     3661 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gaaaactacc 
     3721 cctctagaga tccaccggtc gccaccatga ccgagtacaa gcccacggtg cgcctcgcca 
     3781 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg 
     3841 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac 
     3901 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg 
     3961 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg 
     4021 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc 
     4081 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc 
     4141 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg 
     4201 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg 
     4261 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt 
     4321 gcatgacccg caagcccggt gcctgagcgg ccgcgtcgac aatcaacctc tggattacaa 
     4381 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 
     4441 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 
     4501 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 
     4561 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 
     4621 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 
     4681 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 
     4741 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat 
     4801 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 
     4861 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag 
     4921 tcggatctcc ctttgggccg cctccccgcc tggaattcga gctcggtacc tttaagacca 
     4981 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa 
     5041 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg 
     5101 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 
     5161 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 
     5221 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca 
     5281 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg 
     5341 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 
     5401 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 
     5461 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc 
     5521 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 
     5581 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 
     5641 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt 
     5701 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc 
     5761 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 
     5821 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 
     5881 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 
     5941 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 
     6001 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 
     6061 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 
     6121 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 
     6181 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 
     6241 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta 
     6301 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 
     6361 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 
     6421 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 
     6481 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 
     6541 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 
     6601 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 
     6661 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 
     6721 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 
     6781 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 
     6841 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 
     6901 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 
     6961 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 
     7021 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 
     7081 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 
     7141 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 
     7201 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 
     7261 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 
     7321 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 
     7381 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 
     7441 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 
     7501 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 
     7561 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 
     7621 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 
     7681 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 
     7741 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 
     7801 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 
     7861 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 
     7921 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 
     7981 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 
     8041 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 
     8101 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 
     8161 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 
     8221 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 
     8281 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 
     8341 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 
     8401 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac 
     8461 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgc 
//
