LOCUS       pLV-H1-EF1a-puro        8417 bp    DNA     circular     23-JUN-2008
SOURCE      
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     CDS             6338..7198
                     /gene=Amp(R)
                     /label=Amp(R)
     promoter        7399..7939
                     /gene=pUC ori
                     /label=pUC ori
     promoter        7..235
                     /gene=RSV
                     /label=RSV
     LTR             4943..5177
                     /gene=3'LTR
                     /label=3'LTR
     intron          526..1690
                     /gene=Intron
                     /label=Intron
     rep_origin      complement(5752..6207)
                     /gene=f1 ori
                     /label=f1 ori
     LTR             236..416
                     /gene=5'LTR
                     /label=5'LTR
     polyA_signal    5249..5379
                     /gene=SV40 pA
                     /label=SV40 pA
     misc_feature    5380..5593
                     /gene=SV40 ori
                     /label=SV40 ori
     promoter        2241..2636
                     /gene=human EF1a promoter
                     /label=human EF1a promoter
     intron          2669..3611
                     /gene=intron
                     /label=intron
     stem_loop       1824..1875
                     /gene=shRNA
                     /label=shRNA
     promoter        complement(1876..2104)
                     /gene=H1 promoter
                     /label=H1 promoter
     CDS             3655..4254
                     /gene=Puro(R)
                     
                     /label=Puro(R)
BASE COUNT     1995 a      2139 c      2237 g      2004 t        42 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 
     1861 nnnnnnnnnn nnnnnttttt agagtggtct catacagaac ttataagatt cccaaatcca 
     1921 aagacatttc acgtttatgg tgatttccca gaacacatag cgacatgcaa atattgcagg 
     1981 gcgccactcc cctgtccctc acagccatct tcctgccagg gcgcacgcgc gctgggtgtt 
     2041 cccgcctagt gacactgggc ccgcgattcc ttggagcggg ttgatgacgt cagcgttcga 
     2101 attcggccgg atccaacttt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 
     2161 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 
     2221 aaattcaaaa ttttatcgat gcctccccgt caccaccccc cccaacccgc cccgaccgga 
     2281 gctgagagta attcatacaa aaggactcgc ccctgccttg gggaatccca gggaccgtcg 
     2341 ttaaactccc actaacgtag aacccagaga tcgctgcgtt cccgccccct cacccgcccg 
     2401 ctctcgtcat cactgaggtg gagaagagca tgcgtgaggc tccggtgccc gtcagtgggc 
     2461 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg 
     2521 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct 
     2581 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt 
     2641 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg 
     2701 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacgcccc tggctgcagt 
     2761 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg 
     2821 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc 
     2881 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca 
     2941 tttaaaattt ttgatgatat cctgcgacgc tttttttctg gcaagatagt cttgtaaatg 
     3001 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc 
     3061 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg 
     3121 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta 
     3181 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat 
     3241 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc 
     3301 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat 
     3361 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga 
     3421 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt 
     3481 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc 
     3541 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc 
     3601 ttccatttca ggtgtcgtga aaactacccc tctagagatc caccggtcgc caccatgacc 
     3661 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc 
     3721 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac 
     3781 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 
     3841 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 
     3901 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 
     3961 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 
     4021 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 
     4081 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 
     4141 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 
     4201 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgagcggcc 
     4261 gcgtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact 
     4321 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg 
     4381 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg 
     4441 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa 
     4501 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc 
     4561 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg 
     4621 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat 
     4681 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt 
     4741 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc 
     4801 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg 
     4861 gaattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca 
     4921 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct 
     4981 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 
     5041 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 
     5101 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 
     5161 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc 
     5221 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa 
     5281 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 
     5341 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta 
     5401 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 
     5461 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 
     5521 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt 
     5581 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta 
     5641 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg 
     5701 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct 
     5761 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 
     5821 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 
     5881 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 
     5941 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 
     6001 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 
     6061 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 
     6121 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 
     6181 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac 
     6241 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 
     6301 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 
     6361 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 
     6421 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 
     6481 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag 
     6541 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca 
     6601 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 
     6661 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 
     6721 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 
     6781 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 
     6841 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 
     6901 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 
     6961 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 
     7021 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 
     7081 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 
     7141 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 
     7201 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 
     7261 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 
     7321 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 
     7381 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 
     7441 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 
     7501 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt 
     7561 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 
     7621 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 
     7681 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 
     7741 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 
     7801 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 
     7861 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 
     7921 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 
     7981 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 
     8041 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac 
     8101 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc 
     8161 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 
     8221 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc 
     8281 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca 
     8341 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga 
     8401 acaaaagctg gagctgc 
//
