LOCUS       pLV-mU6-mPGK-red        7741 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     CDS             5662..6522
                     /gene=Amp(R)
     promoter        6723..7263
                     /gene=pUC ori
     promoter        7..235
                     /gene=RSV
     LTR             4267..4501
                     /gene=3'LTR
     intron          526..1690
                     /gene=Intron
     rep_origin      complement(5076..5531)
                     /gene=f1 ori
     LTR             236..416
                     /gene=5'LTR
     polyA_signal    4573..4703
                     /gene=SV40 pA
     misc_feature    4704..4917
                     /gene=SV40 ori
     promoter        2333..2779
                     /gene=mouse PGK promoter
     stem_loop       1824..1875
                     /gene=shRNA
     CDS             2874..3578
                     /gene=RFP
     promoter        complement(1876..2190)
                     /gene=mouse U6 promoter
                     
BASE COUNT     1949 a      1900 c      1987 g      1863 t        42 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 
     1861 nnnnnnnnnn nnnnnttttt aggcttttct ccaagggata tttatagtct caaaacacac 
     1921 aattacttta cagttagggt gagtttcctt ttgtgctgtt ttttaaaata ataatttagt 
     1981 atttgtatct cttatagaaa tccaagccta tcatgtaaaa tgtagctagt attaaaaaga 
     2041 acagattatc tgtcttttat cgcacattaa gcctctatag ttactaggaa atattatatg 
     2101 caaattaacc ggggcagggg agtagccgag cttctcccac aagtctgtgc gagggggccg 
     2161 gcgcgggcct agagatggcg gcgtcggatc gaattcggcc ggatccaact tttaaaagaa 
     2221 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca 
     2281 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg ataattctac 
     2341 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg 
     2401 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg 
     2461 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc 
     2521 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca 
     2581 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt 
     2641 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg 
     2701 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc 
     2761 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct 
     2821 tcctcatctc cgggcctttc gacctgtcta gagtcgagct accggtcgcc accatggtga 
     2881 gcaagggcga ggaggtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca 
     2941 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc 
     3001 agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt 
     3061 ccccccagtt catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt 
     3121 acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg 
     3181 gcggtctggt gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg 
     3241 tgaagatgcg cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg 
     3301 gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc 
     3361 accaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag accatctaca 
     3421 tggccaagaa gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca 
     3481 cctcccacaa cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc 
     3541 acctgttcct gtacggcatg gacgagctgt acaagtaagc ggccgcgtcg acaatcaacc 
     3601 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 
     3661 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 
     3721 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 
     3781 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 
     3841 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 
     3901 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 
     3961 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt 
     4021 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 
     4081 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 
     4141 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc gagctcggta 
     4201 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag 
     4261 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact 
     4321 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 
     4381 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 
     4441 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 
     4501 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca 
     4561 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 
     4621 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 
     4681 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc 
     4741 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 
     4801 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 
     4861 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact 
     4921 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct 
     4981 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc 
     5041 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag 
     5101 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc 
     5161 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc 
     5221 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa 
     5281 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg 
     5341 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac 
     5401 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta 
     5461 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac 
     5521 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt 
     5581 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa 
     5641 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt 
     5701 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat 
     5761 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag 
     5821 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg 
     5881 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata 
     5941 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat 
     6001 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc 
     6061 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg 
     6121 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac 
     6181 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact 
     6241 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa 
     6301 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct 
     6361 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc 
     6421 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga 
     6481 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac 
     6541 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 
     6601 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 
     6661 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 
     6721 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 
     6781 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt 
     6841 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 
     6901 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 
     6961 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 
     7021 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 
     7081 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 
     7141 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 
     7201 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 
     7261 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 
     7321 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt 
     7381 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag 
     7441 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg 
     7501 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc 
     7561 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt 
     7621 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat 
     7681 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg 
     7741 c 
//
