LOCUS       pLV-mU6-EF1a-red        8617 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     CDS             6538..7398
                     /gene=Amp(R)
     promoter        7599..8139
                     /gene=pUC ori
     promoter        7..235
                     /gene=RSV
     LTR             5143..5377
                     /gene=3'LTR
     intron          526..1690
                     /gene=Intron
     rep_origin      complement(5952..6407)
                     /gene=f1 ori
     LTR             236..416
                     /gene=5'LTR
     polyA_signal    5449..5579
                     /gene=SV40 pA
     misc_feature    5580..5793
                     /gene=SV40 ori
     promoter        2333..2728
                     /gene=human EF1a promoter
     intron          2761..3703
                     /gene=intron
     promoter        complement(1876..2190)
                     /gene=mouse U6 promoter
     stem_loop       1824..1875
                     /gene=shRNA
     CDS             3750..4454
                     /gene=RFP
                     
BASE COUNT     2107 a      2124 c      2264 g      2080 t        42 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 
     1861 nnnnnnnnnn nnnnnttttt aggcttttct ccaagggata tttatagtct caaaacacac 
     1921 aattacttta cagttagggt gagtttcctt ttgtgctgtt ttttaaaata ataatttagt 
     1981 atttgtatct cttatagaaa tccaagccta tcatgtaaaa tgtagctagt attaaaaaga 
     2041 acagattatc tgtcttttat cgcacattaa gcctctatag ttactaggaa atattatatg 
     2101 caaattaacc ggggcagggg agtagccgag cttctcccac aagtctgtgc gagggggccg 
     2161 gcgcgggcct agagatggcg gcgtcggatc gaattcggcc ggatccaact tttaaaagaa 
     2221 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca 
     2281 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg atgcctcccc 
     2341 gtcaccaccc cccccaaccc gccccgaccg gagctgagag taattcatac aaaaggactc 
     2401 gcccctgcct tggggaatcc cagggaccgt cgttaaactc ccactaacgt agaacccaga 
     2461 gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc atcactgagg tggagaagag 
     2521 catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 
     2581 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac 
     2641 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 
     2701 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 
     2761 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg 
     2821 ccttgaatta cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt 
     2881 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg 
     2941 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc 
     3001 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgat atcctgcgac 
     3061 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc 
     3121 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag 
     3181 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc 
     3241 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc 
     3301 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag 
     3361 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa 
     3421 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc 
     3481 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg 
     3541 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg 
     3601 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct 
     3661 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gaaaactacc 
     3721 cctctagagt cgagctaccg gtcgccacca tggtgagcaa gggcgaggag gtcatcaaag 
     3781 agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg 
     3841 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca 
     3901 agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca 
     3961 aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccgagg 
     4021 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc gtgacccagg 
     4081 actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc accaacttcc 
     4141 cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc 
     4201 tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggccctgaag ctgaaggacg 
     4261 gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc gtgcaactgc 
     4321 ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgag gactacacca 
     4381 tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgtac ggcatggacg 
     4441 agctgtacaa gtaagcggcc gcgtcgacaa tcaacctctg gattacaaaa tttgtgaaag 
     4501 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat 
     4561 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc 
     4621 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg 
     4681 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct 
     4741 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct 
     4801 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg 
     4861 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac 
     4921 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct 
     4981 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct 
     5041 ttgggccgcc tccccgcctg gaattcgagc tcggtacctt taagaccaat gacttacaag 
     5101 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac 
     5161 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc 
     5221 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 
     5281 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 
     5341 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt 
     5401 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt 
     5461 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 
     5521 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 
     5581 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 
     5641 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 
     5701 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa 
     5761 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga 
     5821 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag 
     5881 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa 
     5941 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 
     6001 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 
     6061 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 
     6121 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 
     6181 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 
     6241 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 
     6301 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 
     6361 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt 
     6421 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 
     6481 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 
     6541 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 
     6601 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 
     6661 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 
     6721 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 
     6781 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 
     6841 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 
     6901 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 
     6961 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 
     7021 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 
     7081 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 
     7141 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 
     7201 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 
     7261 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 
     7321 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 
     7381 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 
     7441 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 
     7501 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 
     7561 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 
     7621 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 
     7681 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc 
     7741 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 
     7801 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 
     7861 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 
     7921 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 
     7981 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 
     8041 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 
     8101 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 
     8161 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 
     8221 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 
     8281 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 
     8341 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 
     8401 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc 
     8461 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt 
     8521 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca 
     8581 attaaccctc actaaaggga acaaaagctg gagctgc 
//
