LOCUS       pLV-mU6-CMV-red         7821 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     CDS             5742..6602
                     /gene=Amp(R)
     promoter        6803..7343
                     /gene=pUC ori
     promoter        7..235
                     /gene=RSV
     LTR             4347..4581
                     /gene=3'LTR
     intron          526..1690
                     /gene=Intron
     rep_origin      complement(5156..5611)
                     /gene=f1 ori
     LTR             236..416
                     /gene=5'LTR
     polyA_signal    4653..4783
                     /gene=SV40 pA
     misc_feature    4784..4997
                     /gene=SV40 ori
     promoter        2340..2871
                     /gene=CMV
     stem_loop       1824..1875
                     /gene=shRNA
     CDS             2954..3658
                     /gene=RFP
     promoter        complement(1876..2190)
                     /gene=mouse U6 promoter
                     
BASE COUNT     2016 a      1884 c      1970 g      1909 t        42 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 
     1861 nnnnnnnnnn nnnnnttttt aggcttttct ccaagggata tttatagtct caaaacacac 
     1921 aattacttta cagttagggt gagtttcctt ttgtgctgtt ttttaaaata ataatttagt 
     1981 atttgtatct cttatagaaa tccaagccta tcatgtaaaa tgtagctagt attaaaaaga 
     2041 acagattatc tgtcttttat cgcacattaa gcctctatag ttactaggaa atattatatg 
     2101 caaattaacc ggggcagggg agtagccgag cttctcccac aagtctgtgc gagggggccg 
     2161 gcgcgggcct agagatggcg gcgtcggatc gaattcggcc ggatccaact tttaaaagaa 
     2221 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca 
     2281 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg ataagcttgg 
     2341 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 
     2401 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 
     2461 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 
     2521 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 
     2581 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 
     2641 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac 
     2701 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa 
     2761 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt 
     2821 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc 
     2881 tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta gagtcgagct 
     2941 accggtcgcc accatggtga gcaagggcga ggaggtcatc aaagagttca tgcgcttcaa 
     3001 ggtgcgcatg gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg 
     3061 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc 
     3121 cttcgcctgg gacatcctgt ccccccagtt catgtacggc tccaaggcgt acgtgaagca 
     3181 ccccgccgac atccccgatt acaagaagct gtccttcccc gagggcttca agtgggagcg 
     3241 cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc caggactcct ccctgcagga 
     3301 cggcacgctg atctacaagg tgaagatgcg cggcaccaac ttcccccccg acggccccgt 
     3361 aatgcagaag aagaccatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg 
     3421 cgtgctgaag ggcgagatcc accaggccct gaagctgaag gacggcggcc actacctggt 
     3481 ggagttcaag accatctaca tggccaagaa gcccgtgcaa ctgcccggct actactacgt 
     3541 ggacaccaag ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga 
     3601 gcgctccgag ggccgccacc acctgttcct gtacggcatg gacgagctgt acaagtaagc 
     3661 ggccgcgtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 
     3721 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 
     3781 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 
     3841 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 
     3901 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 
     3961 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 
     4021 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt 
     4081 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 
     4141 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 
     4201 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg 
     4261 cctggaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta 
     4321 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag 
     4381 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct 
     4441 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 
     4501 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 
     4561 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac 
     4621 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt 
     4681 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 
     4741 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc 
     4801 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 
     4861 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 
     4921 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag 
     4981 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc 
     5041 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa 
     5101 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg 
     5161 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 
     5221 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 
     5281 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 
     5341 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 
     5401 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 
     5461 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 
     5521 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 
     5581 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg 
     5641 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 
     5701 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc 
     5761 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 
     5821 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac 
     5881 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga 
     5941 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag 
     6001 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca 
     6061 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca 
     6121 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa 
     6181 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc 
     6241 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa 
     6301 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag 
     6361 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct 
     6421 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac 
     6481 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa 
     6541 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt 
     6601 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat 
     6661 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 
     6721 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 
     6781 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 
     6841 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 
     6901 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact 
     6961 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 
     7021 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 
     7081 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 
     7141 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 
     7201 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 
     7261 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 
     7321 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 
     7381 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 
     7441 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc 
     7501 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac 
     7561 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact 
     7621 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc 
     7681 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat 
     7741 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa 
     7801 gggaacaaaa gctggagctg c 
//
