LOCUS       pLV-hU6-mPGK-red        7690 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     CDS             5611..6471
                     /gene=Amp(R)
     promoter        6672..7212
                     /gene=pUC ori
     promoter        7..235
                     /gene=RSV
     LTR             4216..4450
                     /gene=3'LTR
     intron          526..1690
                     /gene=Intron
     rep_origin      complement(5025..5480)
                     /gene=f1 ori
     LTR             236..416
                     /gene=5'LTR
     polyA_signal    4522..4652
                     /gene=SV40 pA
     misc_feature    4653..4866
                     /gene=SV40 ori
     promoter        2282..2728
                     /gene=mouse PGK promoter
     promoter        complement(1876..2139)
                     /gene=human U6 promoter
     stem_loop       1824..1875
                     /gene=shRNA
     CDS             2823..3527
                     /gene=RFP
                     
BASE COUNT     1947 a      1896 c      1952 g      1853 t        42 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 
     1861 nnnnnnnnnn nnnnnttttt ttcgtccttt ccacaagata tataaagcca agaaatcgaa 
     1921 atactttcaa gttacggtaa gcatatgata gtccatttta aaacataatt ttaaaactgc 
     1981 aaactaccca agaaattatt actttctacg tcacgtattt tgtactaata tctttgtgtt 
     2041 tacagtcaaa ttaattctaa ttatctctct aacagccttg tatcgtatat gcaaatatga 
     2101 aggaatcatg ggaaataggc cctcttcctg cccgaccttg aattcggccg gatccaactt 
     2161 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag 
     2221 caacagacat acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcga 
     2281 taattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca 
     2341 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc 
     2401 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct 
     2461 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg 
     2521 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg 
     2581 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg 
     2641 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc 
     2701 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg 
     2761 ttctcctctt cctcatctcc gggcctttcg acctgtctag agtcgagcta ccggtcgcca 
     2821 ccatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg 
     2881 agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg 
     2941 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg 
     3001 acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca 
     3061 tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact 
     3121 tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga 
     3181 tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga 
     3241 agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg 
     3301 gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga 
     3361 ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc 
     3421 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg 
     3481 gccgccacca cctgttcctg tacggcatgg acgagctgta caagtaagcg gccgcgtcga 
     3541 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 
     3601 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 
     3661 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 
     3721 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 
     3781 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 
     3841 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 
     3901 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct 
     3961 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 
     4021 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 
     4081 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattcg 
     4141 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 
     4201 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt 
     4261 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta 
     4321 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc 
     4381 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa 
     4441 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa 
     4501 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc 
     4561 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 
     4621 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc 
     4681 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 
     4741 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 
     4801 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc 
     4861 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact 
     4921 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac 
     4981 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg 
     5041 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 
     5101 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 
     5161 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 
     5221 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac 
     5281 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 
     5341 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat 
     5401 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa 
     5461 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt 
     5521 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 
     5581 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt 
     5641 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa 
     5701 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac 
     5761 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt 
     5821 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt 
     5881 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat 
     5941 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac 
     6001 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg 
     6061 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc 
     6121 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa 
     6181 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag 
     6241 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct 
     6301 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat 
     6361 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa 
     6421 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac 
     6481 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 
     6541 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 
     6601 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 
     6661 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 
     6721 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 
     6781 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 
     6841 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 
     6901 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 
     6961 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 
     7021 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 
     7081 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 
     7141 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 
     7201 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 
     7261 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 
     7321 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag 
     7381 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc 
     7441 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc 
     7501 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac 
     7561 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga 
     7621 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag 
     7681 ctggagctgc 
//
