LOCUS       pLV-hU6-EF1a-red        8566 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     CDS             6487..7347
                     /gene=Amp(R)
     promoter        7548..8088
                     /gene=pUC ori
     promoter        7..235
                     /gene=RSV
     LTR             5092..5326
                     /gene=3'LTR
     intron          526..1690
                     /gene=Intron
     rep_origin      complement(5901..6356)
                     /gene=f1 ori
     LTR             236..416
                     /gene=5'LTR
     polyA_signal    5398..5528
                     /gene=SV40 pA
     misc_feature    5529..5742
                     /gene=SV40 ori
     promoter        2282..2677
                     /gene=human EF1a promoter
     intron          2710..3652
                     /gene=intron
     stem_loop       1824..1875
                     /gene=shRNA
     CDS             3699..4403
                     /gene=RFP
     promoter        complement(1876..2139)
                     /gene=human U6 promoter
                     
BASE COUNT     2105 a      2120 c      2229 g      2070 t        42 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 
     1861 nnnnnnnnnn nnnnnttttt ttcgtccttt ccacaagata tataaagcca agaaatcgaa 
     1921 atactttcaa gttacggtaa gcatatgata gtccatttta aaacataatt ttaaaactgc 
     1981 aaactaccca agaaattatt actttctacg tcacgtattt tgtactaata tctttgtgtt 
     2041 tacagtcaaa ttaattctaa ttatctctct aacagccttg tatcgtatat gcaaatatga 
     2101 aggaatcatg ggaaataggc cctcttcctg cccgaccttg aattcggccg gatccaactt 
     2161 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag 
     2221 caacagacat acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcga 
     2281 tgcctccccg tcaccacccc ccccaacccg ccccgaccgg agctgagagt aattcataca 
     2341 aaaggactcg cccctgcctt ggggaatccc agggaccgtc gttaaactcc cactaacgta 
     2401 gaacccagag atcgctgcgt tcccgccccc tcacccgccc gctctcgtca tcactgaggt 
     2461 ggagaagagc atgcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 
     2521 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc 
     2581 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 
     2641 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 
     2701 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc 
     2761 ccttgcgtgc cttgaattac ttccacgccc ctggctgcag tacgtgattc ttgatcccga 
     2821 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc 
     2881 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca 
     2941 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgata 
     3001 tcctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac 
     3061 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg 
     3121 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc 
     3181 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc 
     3241 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc 
     3301 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac 
     3361 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg 
     3421 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg 
     3481 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag 
     3541 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg 
     3601 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg 
     3661 aaaactaccc ctctagagtc gagctaccgg tcgccaccat ggtgagcaag ggcgaggagg 
     3721 tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt 
     3781 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga 
     3841 aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt 
     3901 acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct 
     3961 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg 
     4021 tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca 
     4081 ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca 
     4141 ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc 
     4201 tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg 
     4261 tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg 
     4321 actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctgtacg 
     4381 gcatggacga gctgtacaag taagcggccg cgtcgacaat caacctctgg attacaaaat 
     4441 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc 
     4501 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt 
     4561 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg 
     4621 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg 
     4681 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc 
     4741 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt 
     4801 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct 
     4861 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg 
     4921 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg 
     4981 gatctccctt tgggccgcct ccccgcctgg aattcgagct cggtaccttt aagaccaatg 
     5041 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg 
     5101 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta 
     5161 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 
     5221 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 
     5281 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt 
     5341 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac 
     5401 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 
     5461 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 
     5521 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca 
     5581 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 
     5641 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga 
     5701 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca 
     5761 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 
     5821 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 
     5881 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 
     5941 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 
     6001 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 
     6061 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 
     6121 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 
     6181 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 
     6241 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 
     6301 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt 
     6361 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca 
     6421 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 
     6481 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc 
     6541 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg 
     6601 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt 
     6661 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta 
     6721 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat 
     6781 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga 
     6841 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca 
     6901 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact 
     6961 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc 
     7021 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact 
     7081 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt 
     7141 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt 
     7201 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt 
     7261 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 
     7321 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag 
     7381 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 
     7441 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 
     7501 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 
     7561 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 
     7621 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg 
     7681 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 
     7741 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 
     7801 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 
     7861 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 
     7921 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 
     7981 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 
     8041 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 
     8101 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 
     8161 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag 
     8221 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 
     8281 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 
     8341 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 
     8401 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg 
     8461 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc 
     8521 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgc 
//
