LOCUS       pLV-hU6-CMV-red         7770 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     CDS             5691..6551
                     /gene=Amp(R)
     promoter        6752..7292
                     /gene=pUC ori
     promoter        7..235
                     /gene=RSV
     LTR             4296..4530
                     /gene=3'LTR
     intron          526..1690
                     /gene=Intron
     rep_origin      complement(5105..5560)
                     /gene=f1 ori
     LTR             236..416
                     /gene=5'LTR
     polyA_signal    4602..4732
                     /gene=SV40 pA
     misc_feature    4733..4946
                     /gene=SV40 ori
     promoter        2289..2820
                     /gene=CMV
     stem_loop       1824..1875
                     /gene=shRNA
     CDS             2903..3607
                     /gene=RFP
     promoter        complement(1876..2139)
                     /gene=human U6 promoter
                     
BASE COUNT     2014 a      1880 c      1935 g      1899 t        42 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 
     1861 nnnnnnnnnn nnnnnttttt ttcgtccttt ccacaagata tataaagcca agaaatcgaa 
     1921 atactttcaa gttacggtaa gcatatgata gtccatttta aaacataatt ttaaaactgc 
     1981 aaactaccca agaaattatt actttctacg tcacgtattt tgtactaata tctttgtgtt 
     2041 tacagtcaaa ttaattctaa ttatctctct aacagccttg tatcgtatat gcaaatatga 
     2101 aggaatcatg ggaaataggc cctcttcctg cccgaccttg aattcggccg gatccaactt 
     2161 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag 
     2221 caacagacat acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcga 
     2281 taagcttggg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc 
     2341 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg 
     2401 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat 
     2461 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc 
     2521 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta 
     2581 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag 
     2641 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt 
     2701 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa 
     2761 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt 
     2821 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgactctag 
     2881 agtcgagcta ccggtcgcca ccatggtgag caagggcgag gaggtcatca aagagttcat 
     2941 gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga 
     3001 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg 
     3061 ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta 
     3121 cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa 
     3181 gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc 
     3241 cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga 
     3301 cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc 
     3361 ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca 
     3421 ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta 
     3481 ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga 
     3541 acagtacgag cgctccgagg gccgccacca cctgttcctg tacggcatgg acgagctgta 
     3601 caagtaagcg gccgcgtcga caatcaacct ctggattaca aaatttgtga aagattgact 
     3661 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 
     3721 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg 
     3781 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg 
     3841 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg 
     3901 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc 
     3961 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg 
     4021 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc 
     4081 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct 
     4141 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc 
     4201 gcctccccgc ctggaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg 
     4261 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac 
     4321 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct 
     4381 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 
     4441 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 
     4501 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt 
     4561 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt 
     4621 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 
     4681 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc 
     4741 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 
     4801 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 
     4861 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc 
     4921 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa 
     4981 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt 
     5041 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa 
     5101 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 
     5161 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 
     5221 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga 
     5281 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 
     5341 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 
     5401 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 
     5461 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 
     5521 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa 
     5581 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 
     5641 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc 
     5701 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 
     5761 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt 
     5821 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt 
     5881 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg 
     5941 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact 
     6001 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg 
     6061 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 
     6121 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg 
     6181 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa 
     6241 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac 
     6301 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 
     6361 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca 
     6421 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 
     6481 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 
     6541 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 
     6601 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 
     6661 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 
     6721 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 
     6781 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 
     6841 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact 
     6901 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 
     6961 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 
     7021 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 
     7081 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 
     7141 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 
     7201 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 
     7261 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 
     7321 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 
     7381 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc 
     7441 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa 
     7501 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt 
     7561 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt 
     7621 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg 
     7681 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc 
     7741 ctcactaaag ggaacaaaag ctggagctgc 
//
