LOCUS       pLV-H1-mPGK-red         7649 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     CDS             5570..6430
                     /gene=Amp(R)
     promoter        6631..7171
                     /gene=pUC ori
     promoter        7..235
                     /gene=RSV
     LTR             4175..4409
                     /gene=3'LTR
     intron          526..1690
                     /gene=Intron
     rep_origin      complement(4984..5439)
                     /gene=f1 ori
     LTR             236..416
                     /gene=5'LTR
     polyA_signal    4481..4611
                     /gene=SV40 pA
     misc_feature    4612..4825
                     /gene=SV40 ori
     promoter        2241..2687
                     /gene=mouse PGK promoter
     stem_loop       1824..1875
                     /gene=shRNA
     CDS             2782..3486
                     /gene=RFP
     promoter        complement(1876..2104)
                     /gene=H1 promoter
                     
BASE COUNT     1909 a      1909 c      1972 g      1817 t        42 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 
     1861 nnnnnnnnnn nnnnnttttt agagtggtct catacagaac ttataagatt cccaaatcca 
     1921 aagacatttc acgtttatgg tgatttccca gaacacatag cgacatgcaa atattgcagg 
     1981 gcgccactcc cctgtccctc acagccatct tcctgccagg gcgcacgcgc gctgggtgtt 
     2041 cccgcctagt gacactgggc ccgcgattcc ttggagcggg ttgatgacgt cagcgttcga 
     2101 attcggccgg atccaacttt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 
     2161 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 
     2221 aaattcaaaa ttttatcgat aattctaccg ggtaggggag gcgcttttcc caaggcagtc 
     2281 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc 
     2341 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt 
     2401 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc 
     2461 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac 
     2521 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt 
     2581 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct 
     2641 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa 
     2701 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgtctaga 
     2761 gtcgagctac cggtcgccac catggtgagc aagggcgagg aggtcatcaa agagttcatg 
     2821 cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag 
     2881 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc 
     2941 cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac 
     3001 gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag 
     3061 tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc 
     3121 ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac 
     3181 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc 
     3241 cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac 
     3301 tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac 
     3361 tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa 
     3421 cagtacgagc gctccgaggg ccgccaccac ctgttcctgt acggcatgga cgagctgtac 
     3481 aagtaagcgg ccgcgtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg 
     3541 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt 
     3601 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc 
     3661 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt 
     3721 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga 
     3781 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct 
     3841 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga 
     3901 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct 
     3961 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc 
     4021 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg 
     4081 cctccccgcc tggaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt 
     4141 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 
     4201 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 
     4261 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 
     4321 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 
     4381 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 
     4441 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 
     4501 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 
     4561 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 
     4621 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 
     4681 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 
     4741 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct 
     4801 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa 
     4861 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta 
     4921 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat 
     4981 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 
     5041 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 
     5101 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 
     5161 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 
     5221 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 
     5281 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 
     5341 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 
     5401 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa 
     5461 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 
     5521 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca 
     5581 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca 
     5641 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta 
     5701 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt 
     5761 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc 
     5821 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc 
     5881 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc 
     5941 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa 
     6001 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga 
     6061 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat 
     6121 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca 
     6181 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc 
     6241 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat 
     6301 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag 
     6361 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa 
     6421 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca 
     6481 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 
     6541 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 
     6601 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 
     6661 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 
     6721 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 
     6781 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 
     6841 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 
     6901 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 
     6961 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 
     7021 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 
     7081 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 
     7141 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 
     7201 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 
     7261 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 
     7321 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat 
     7381 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 
     7441 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 
     7501 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 
     7561 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc 
     7621 tcactaaagg gaacaaaagc tggagctgc 
//
