LOCUS       pLV-H1-EF1a-red         8525 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     CDS             6446..7306
                     /gene=Amp(R)
     promoter        7507..8047
                     /gene=pUC ori
     promoter        7..235
                     /gene=RSV
     LTR             5051..5285
                     /gene=3'LTR
     intron          526..1690
                     /gene=Intron
     rep_origin      complement(5860..6315)
                     /gene=f1 ori
     LTR             236..416
                     /gene=5'LTR
     polyA_signal    5357..5487
                     /gene=SV40 pA
     misc_feature    5488..5701
                     /gene=SV40 ori
     promoter        2241..2636
                     /gene=human EF1a promoter
     intron          2669..3611
                     /gene=intron
     stem_loop       1824..1875
                     /gene=shRNA
     CDS             3658..4362
                     /gene=RFP
     promoter        complement(1876..2104)
                     /gene=H1 promoter
                     
BASE COUNT     2067 a      2133 c      2249 g      2034 t        42 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 
     1861 nnnnnnnnnn nnnnnttttt agagtggtct catacagaac ttataagatt cccaaatcca 
     1921 aagacatttc acgtttatgg tgatttccca gaacacatag cgacatgcaa atattgcagg 
     1981 gcgccactcc cctgtccctc acagccatct tcctgccagg gcgcacgcgc gctgggtgtt 
     2041 cccgcctagt gacactgggc ccgcgattcc ttggagcggg ttgatgacgt cagcgttcga 
     2101 attcggccgg atccaacttt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 
     2161 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 
     2221 aaattcaaaa ttttatcgat gcctccccgt caccaccccc cccaacccgc cccgaccgga 
     2281 gctgagagta attcatacaa aaggactcgc ccctgccttg gggaatccca gggaccgtcg 
     2341 ttaaactccc actaacgtag aacccagaga tcgctgcgtt cccgccccct cacccgcccg 
     2401 ctctcgtcat cactgaggtg gagaagagca tgcgtgaggc tccggtgccc gtcagtgggc 
     2461 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg 
     2521 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct 
     2581 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt 
     2641 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg 
     2701 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacgcccc tggctgcagt 
     2761 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg 
     2821 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc 
     2881 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca 
     2941 tttaaaattt ttgatgatat cctgcgacgc tttttttctg gcaagatagt cttgtaaatg 
     3001 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc 
     3061 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg 
     3121 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta 
     3181 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat 
     3241 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc 
     3301 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat 
     3361 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga 
     3421 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt 
     3481 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc 
     3541 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc 
     3601 ttccatttca ggtgtcgtga aaactacccc tctagagtcg agctaccggt cgccaccatg 
     3661 gtgagcaagg gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg catggagggc 
     3721 tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc 
     3781 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc 
     3841 ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc 
     3901 gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag 
     3961 gacggcggtc tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac 
     4021 aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc 
     4081 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag 
     4141 atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc 
     4201 tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac 
     4261 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc 
     4321 caccacctgt tcctgtacgg catggacgag ctgtacaagt aagcggccgc gtcgacaatc 
     4381 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 
     4441 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 
     4501 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 
     4561 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 
     4621 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 
     4681 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 
     4741 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct 
     4801 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 
     4861 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 
     4921 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc 
     4981 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga 
     5041 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg 
     5101 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 
     5161 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 
     5221 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 
     5281 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat 
     5341 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag 
     5401 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 
     5461 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc 
     5521 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 
     5581 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 
     5641 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct 
     5701 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc 
     5761 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc 
     5821 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat 
     5881 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag 
     5941 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc 
     6001 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc 
     6061 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt 
     6121 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa 
     6181 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg 
     6241 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat 
     6301 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 
     6361 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 
     6421 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc 
     6481 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa 
     6541 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg 
     6601 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt 
     6661 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg 
     6721 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac 
     6781 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc 
     6841 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa 
     6901 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc 
     6961 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt 
     7021 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga 
     7081 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa 
     7141 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa 
     7201 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa 
     7261 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt 
     7321 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 
     7381 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 
     7441 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 
     7501 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 
     7561 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 
     7621 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 
     7681 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 
     7741 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 
     7801 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 
     7861 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 
     7921 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 
     7981 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 
     8041 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 
     8101 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 
     8161 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 
     8221 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg 
     8281 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga 
     8341 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat 
     8401 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag 
     8461 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga 
     8521 gctgc 
//
