LOCUS       pLV-H1-CMV-red          7729 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     CDS             5650..6510
                     /gene=Amp(R)
     promoter        6711..7251
                     /gene=pUC ori
     promoter        7..235
                     /gene=RSV
     LTR             4255..4489
                     /gene=3'LTR
     intron          526..1690
                     /gene=Intron
     rep_origin      complement(5064..5519)
                     /gene=f1 ori
     LTR             236..416
                     /gene=5'LTR
     polyA_signal    4561..4691
                     /gene=SV40 pA
     misc_feature    4692..4905
                     /gene=SV40 ori
     promoter        2248..2779
                     /gene=CMV
     promoter        complement(1876..2104)
                     /gene=H1 promoter
     stem_loop       1824..1875
                     /gene=shRNA
     CDS             2862..3566
                     /gene=RFP
                     
BASE COUNT     1976 a      1893 c      1955 g      1863 t        42 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 
     1861 nnnnnnnnnn nnnnnttttt agagtggtct catacagaac ttataagatt cccaaatcca 
     1921 aagacatttc acgtttatgg tgatttccca gaacacatag cgacatgcaa atattgcagg 
     1981 gcgccactcc cctgtccctc acagccatct tcctgccagg gcgcacgcgc gctgggtgtt 
     2041 cccgcctagt gacactgggc ccgcgattcc ttggagcggg ttgatgacgt cagcgttcga 
     2101 attcggccgg atccaacttt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 
     2161 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 
     2221 aaattcaaaa ttttatcgat aagcttggga gttccgcgtt acataactta cggtaaatgg 
     2281 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc 
     2341 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac 
     2401 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa 
     2461 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac 
     2521 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta 
     2581 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga 
     2641 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa 
     2701 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag 
     2761 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca 
     2821 tagaagacac cgactctaga gtcgagctac cggtcgccac catggtgagc aagggcgagg 
     2881 aggtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg 
     2941 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc 
     3001 tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca 
     3061 tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt 
     3121 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga 
     3181 ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg 
     3241 gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct 
     3301 ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga 
     3361 agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc 
     3421 ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg 
     3481 aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgt 
     3541 acggcatgga cgagctgtac aagtaagcgg ccgcgtcgac aatcaacctc tggattacaa 
     3601 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 
     3661 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 
     3721 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 
     3781 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 
     3841 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 
     3901 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 
     3961 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat 
     4021 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 
     4081 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag 
     4141 tcggatctcc ctttgggccg cctccccgcc tggaattcga gctcggtacc tttaagacca 
     4201 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa 
     4261 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg 
     4321 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 
     4381 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 
     4441 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca 
     4501 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg 
     4561 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 
     4621 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 
     4681 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc 
     4741 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 
     4801 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 
     4861 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt 
     4921 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc 
     4981 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 
     5041 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 
     5101 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 
     5161 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 
     5221 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 
     5281 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 
     5341 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 
     5401 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 
     5461 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta 
     5521 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 
     5581 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 
     5641 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 
     5701 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 
     5761 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 
     5821 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 
     5881 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 
     5941 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 
     6001 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 
     6061 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 
     6121 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 
     6181 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 
     6241 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 
     6301 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 
     6361 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 
     6421 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 
     6481 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 
     6541 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 
     6601 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 
     6661 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 
     6721 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 
     6781 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 
     6841 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 
     6901 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 
     6961 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 
     7021 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 
     7081 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 
     7141 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 
     7201 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 
     7261 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 
     7321 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 
     7381 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 
     7441 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 
     7501 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 
     7561 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 
     7621 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac 
     7681 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgc 
//
