LOCUS       pLV-mU6-mPGK-green        7754 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     CDS             5675..6535
                     /gene=Amp(R)
     promoter        6736..7276
                     /gene=pUC ori
     promoter        7..235
                     /gene=RSV
     LTR             4280..4514
                     /gene=3'LTR
     intron          526..1690
                     /gene=Intron
     rep_origin      complement(5089..5544)
                     /gene=f1 ori
     LTR             236..416
                     /gene=5'LTR
     polyA_signal    4586..4716
                     /gene=SV40 pA
     misc_feature    4717..4930
                     /gene=SV40 ori
     promoter        2333..2779
                     /gene=mouse PGK promoter
     CDS             2871..3590
                     /gene=GFP
     stem_loop       1824..1875
                     /gene=shRNA
     promoter        complement(1876..2190)
                     /gene=mouse U6 promoter
                     
BASE COUNT     1965 a      1912 c      1974 g      1861 t        42 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 
     1861 nnnnnnnnnn nnnnnttttt aggcttttct ccaagggata tttatagtct caaaacacac 
     1921 aattacttta cagttagggt gagtttcctt ttgtgctgtt ttttaaaata ataatttagt 
     1981 atttgtatct cttatagaaa tccaagccta tcatgtaaaa tgtagctagt attaaaaaga 
     2041 acagattatc tgtcttttat cgcacattaa gcctctatag ttactaggaa atattatatg 
     2101 caaattaacc ggggcagggg agtagccgag cttctcccac aagtctgtgc gagggggccg 
     2161 gcgcgggcct agagatggcg gcgtcggatc gaattcggcc ggatccaact tttaaaagaa 
     2221 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca 
     2281 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg ataattctac 
     2341 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg 
     2401 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg 
     2461 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc 
     2521 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca 
     2581 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt 
     2641 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg 
     2701 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc 
     2761 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct 
     2821 tcctcatctc cgggcctttc gacctgtcta gagatccacc ggtcgccacc atggtgagca 
     2881 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 
     2941 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga 
     3001 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 
     3061 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact 
     3121 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg 
     3181 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 
     3241 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 
     3301 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg 
     3361 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc 
     3421 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca 
     3481 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt 
     3541 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg 
     3601 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg 
     3661 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt 
     3721 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg 
     3781 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc 
     3841 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc 
     3901 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc 
     3961 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc 
     4021 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg 
     4081 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc 
     4141 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa 
     4201 ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 
     4261 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct 
     4321 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 
     4381 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 
     4441 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 
     4501 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 
     4561 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata 
     4621 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 
     4681 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact 
     4741 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 
     4801 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 
     4861 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt 
     4921 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc 
     4981 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc 
     5041 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta 
     5101 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 
     5161 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 
     5221 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc 
     5281 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat 
     5341 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 
     5401 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc 
     5461 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta 
     5521 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc 
     5581 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 
     5641 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc 
     5701 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt 
     5761 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct 
     5821 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac 
     5881 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact 
     5941 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa 
     6001 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga 
     6061 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt 
     6121 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga 
     6181 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg 
     6241 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat 
     6301 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat 
     6361 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc 
     6421 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga 
     6481 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc 
     6541 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag 
     6601 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 
     6661 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 
     6721 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 
     6781 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 
     6841 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 
     6901 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 
     6961 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 
     7021 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 
     7081 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 
     7141 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 
     7201 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 
     7261 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 
     7321 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 
     7381 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac 
     7441 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct 
     7501 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc 
     7561 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt 
     7621 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac 
     7681 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca 
     7741 aaagctggag ctgc 
//
