LOCUS       pLV-mU6-EF1a-green        8630 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     CDS             6551..7411
                     /gene=Amp(R)
     promoter        7612..8152
                     /gene=pUC ori
     promoter        7..235
                     /gene=RSV
     LTR             5156..5390
                     /gene=3'LTR
     intron          526..1690
                     /gene=Intron
     rep_origin      complement(5965..6420)
                     /gene=f1 ori
     LTR             236..416
                     /gene=5'LTR
     polyA_signal    5462..5592
                     /gene=SV40 pA
     misc_feature    5593..5806
                     /gene=SV40 ori
     promoter        2333..2728
                     /gene=human EF1a promoter
     intron          2761..3703
                     /gene=intron
     CDS             3747..4466
                     /gene=GFP
     promoter        complement(1876..2190)
                     /gene=mouse U6 promoter
     stem_loop       1824..1875
                     /gene=shRNA
                     
BASE COUNT     2123 a      2136 c      2251 g      2078 t        42 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 
     1861 nnnnnnnnnn nnnnnttttt aggcttttct ccaagggata tttatagtct caaaacacac 
     1921 aattacttta cagttagggt gagtttcctt ttgtgctgtt ttttaaaata ataatttagt 
     1981 atttgtatct cttatagaaa tccaagccta tcatgtaaaa tgtagctagt attaaaaaga 
     2041 acagattatc tgtcttttat cgcacattaa gcctctatag ttactaggaa atattatatg 
     2101 caaattaacc ggggcagggg agtagccgag cttctcccac aagtctgtgc gagggggccg 
     2161 gcgcgggcct agagatggcg gcgtcggatc gaattcggcc ggatccaact tttaaaagaa 
     2221 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca 
     2281 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg atgcctcccc 
     2341 gtcaccaccc cccccaaccc gccccgaccg gagctgagag taattcatac aaaaggactc 
     2401 gcccctgcct tggggaatcc cagggaccgt cgttaaactc ccactaacgt agaacccaga 
     2461 gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc atcactgagg tggagaagag 
     2521 catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 
     2581 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac 
     2641 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 
     2701 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 
     2761 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg 
     2821 ccttgaatta cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt 
     2881 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg 
     2941 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc 
     3001 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgat atcctgcgac 
     3061 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc 
     3121 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag 
     3181 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc 
     3241 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc 
     3301 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag 
     3361 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa 
     3421 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc 
     3481 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg 
     3541 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg 
     3601 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct 
     3661 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gaaaactacc 
     3721 cctctagaga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg 
     3781 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg 
     3841 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg 
     3901 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct 
     3961 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag 
     4021 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 
     4081 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 
     4141 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct 
     4201 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca 
     4261 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 
     4321 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc 
     4381 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 
     4441 tcggcatgga cgagctgtac aagtaaagcg gccgcgtcga caatcaacct ctggattaca 
     4501 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 
     4561 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 
     4621 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac 
     4681 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca 
     4741 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 
     4801 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg 
     4861 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga 
     4921 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 
     4981 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga 
     5041 gtcggatctc cctttgggcc gcctccccgc ctggaattcg agctcggtac ctttaagacc 
     5101 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga 
     5161 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg 
     5221 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 
     5281 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 
     5341 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 
     5401 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 
     5461 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 
     5521 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 
     5581 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg 
     5641 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 
     5701 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 
     5761 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt 
     5821 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc 
     5881 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt 
     5941 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg 
     6001 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 
     6061 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 
     6121 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 
     6181 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 
     6241 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 
     6301 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa 
     6361 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt 
     6421 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 
     6481 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 
     6541 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 
     6601 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 
     6661 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 
     6721 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 
     6781 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 
     6841 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 
     6901 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 
     6961 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 
     7021 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 
     7081 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 
     7141 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 
     7201 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 
     7261 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 
     7321 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 
     7381 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 
     7441 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 
     7501 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 
     7561 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 
     7621 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 
     7681 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta 
     7741 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 
     7801 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 
     7861 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 
     7921 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 
     7981 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 
     8041 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 
     8101 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 
     8161 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 
     8221 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 
     8281 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 
     8341 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 
     8401 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa 
     8461 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat 
     8521 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta 
     8581 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc 
//
