LOCUS       pLV-mU6-CMV-green        7834 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     CDS             5755..6615
                     /gene=Amp(R)
     promoter        6816..7356
                     /gene=pUC ori
     promoter        7..235
                     /gene=RSV
     LTR             4360..4594
                     /gene=3'LTR
     intron          526..1690
                     /gene=Intron
     rep_origin      complement(5169..5624)
                     /gene=f1 ori
     LTR             236..416
                     /gene=5'LTR
     polyA_signal    4666..4796
                     /gene=SV40 pA
     misc_feature    4797..5010
                     /gene=SV40 ori
     promoter        2340..2871
                     /gene=CMV
     CDS             2951..3670
                     /gene=GFP
     stem_loop       1824..1875
                     /gene=shRNA
     promoter        complement(1876..2190)
                     /gene=mouse U6 promoter
                     
BASE COUNT     2032 a      1896 c      1957 g      1907 t        42 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 
     1861 nnnnnnnnnn nnnnnttttt aggcttttct ccaagggata tttatagtct caaaacacac 
     1921 aattacttta cagttagggt gagtttcctt ttgtgctgtt ttttaaaata ataatttagt 
     1981 atttgtatct cttatagaaa tccaagccta tcatgtaaaa tgtagctagt attaaaaaga 
     2041 acagattatc tgtcttttat cgcacattaa gcctctatag ttactaggaa atattatatg 
     2101 caaattaacc ggggcagggg agtagccgag cttctcccac aagtctgtgc gagggggccg 
     2161 gcgcgggcct agagatggcg gcgtcggatc gaattcggcc ggatccaact tttaaaagaa 
     2221 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca 
     2281 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg ataagcttgg 
     2341 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 
     2401 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 
     2461 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 
     2521 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 
     2581 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 
     2641 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac 
     2701 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa 
     2761 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt 
     2821 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc 
     2881 tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta gagatccacc 
     2941 ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt 
     3001 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga 
     3061 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc 
     3121 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga 
     3181 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg 
     3241 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg 
     3301 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat 
     3361 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa 
     3421 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt 
     3481 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc 
     3541 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga 
     3601 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct 
     3661 gtacaagtaa agcggccgcg tcgacaatca acctctggat tacaaaattt gtgaaagatt 
     3721 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc 
     3781 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg 
     3841 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac 
     3901 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc 
     3961 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc 
     4021 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa 
     4081 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc 
     4141 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc 
     4201 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg 
     4261 ggccgcctcc ccgcctggaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca 
     4321 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc 
     4381 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga 
     4441 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
     4501 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
     4561 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt 
     4621 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca 
     4681 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 
     4741 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc 
     4801 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 
     4861 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 
     4921 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc 
     4981 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg 
     5041 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg 
     5101 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg 
     5161 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 
     5221 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt 
     5281 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 
     5341 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg 
     5401 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 
     5461 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 
     5521 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 
     5581 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg 
     5641 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 
     5701 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 
     5761 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 
     5821 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 
     5881 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 
     5941 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 
     6001 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 
     6061 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 
     6121 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 
     6181 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 
     6241 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 
     6301 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 
     6361 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 
     6421 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 
     6481 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 
     6541 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 
     6601 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 
     6661 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 
     6721 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 
     6781 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 
     6841 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 
     6901 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 
     6961 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 
     7021 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 
     7081 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 
     7141 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 
     7201 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 
     7261 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 
     7321 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 
     7381 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 
     7441 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 
     7501 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 
     7561 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 
     7621 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 
     7681 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 
     7741 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt 
     7801 aaccctcact aaagggaaca aaagctggag ctgc 
//
