LOCUS       pLV-hU6-mPGK-green      7703 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     CDS             5624..6484
                     /gene=Amp(R)
     promoter        6685..7225
                     /gene=pUC ori
     promoter        7..235
                     /gene=RSV
     LTR             4229..4463
                     /gene=3'LTR
     intron          526..1690
                     /gene=Intron
     rep_origin      complement(5038..5493)
                     /gene=f1 ori
     LTR             236..416
                     /gene=5'LTR
     polyA_signal    4535..4665
                     /gene=SV40 pA
     misc_feature    4666..4879
                     /gene=SV40 ori
     promoter        2282..2728
                     /gene=mouse PGK promoter
     CDS             2820..3539
                     /gene=GFP
     promoter        complement(1876..2139)
                     /gene=human U6 promoter
     stem_loop       1824..1875
                     /gene=shRNA
                     
BASE COUNT     1963 a      1908 c      1939 g      1851 t        42 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 
     1861 nnnnnnnnnn nnnnnttttt ttcgtccttt ccacaagata tataaagcca agaaatcgaa 
     1921 atactttcaa gttacggtaa gcatatgata gtccatttta aaacataatt ttaaaactgc 
     1981 aaactaccca agaaattatt actttctacg tcacgtattt tgtactaata tctttgtgtt 
     2041 tacagtcaaa ttaattctaa ttatctctct aacagccttg tatcgtatat gcaaatatga 
     2101 aggaatcatg ggaaataggc cctcttcctg cccgaccttg aattcggccg gatccaactt 
     2161 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag 
     2221 caacagacat acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcga 
     2281 taattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca 
     2341 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc 
     2401 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct 
     2461 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg 
     2521 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg 
     2581 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg 
     2641 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc 
     2701 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg 
     2761 ttctcctctt cctcatctcc gggcctttcg acctgtctag agatccaccg gtcgccacca 
     2821 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg 
     2881 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg 
     2941 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc 
     3001 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc 
     3061 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct 
     3121 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg 
     3181 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca 
     3241 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg 
     3301 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg 
     3361 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact 
     3421 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc 
     3481 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa 
     3541 gcggccgcgt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 
     3601 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 
     3661 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 
     3721 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 
     3781 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 
     3841 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 
     3901 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct 
     3961 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 
     4021 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 
     4081 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 
     4141 cgcctggaat tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct 
     4201 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca 
     4261 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct 
     4321 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 
     4381 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 
     4441 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 
     4501 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg 
     4561 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 
     4621 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg 
     4681 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 
     4741 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 
     4801 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg 
     4861 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg 
     4921 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg 
     4981 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg 
     5041 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 
     5101 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 
     5161 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg 
     5221 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 
     5281 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 
     5341 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 
     5401 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 
     5461 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg 
     5521 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 
     5581 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 
     5641 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 
     5701 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 
     5761 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 
     5821 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca 
     5881 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 
     5941 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 
     6001 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 
     6061 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 
     6121 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 
     6181 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 
     6241 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 
     6301 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 
     6361 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 
     6421 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 
     6481 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 
     6541 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 
     6601 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 
     6661 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 
     6721 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 
     6781 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa 
     6841 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 
     6901 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 
     6961 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 
     7021 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 
     7081 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 
     7141 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 
     7201 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 
     7261 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 
     7321 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag 
     7381 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa 
     7441 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga 
     7501 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc 
     7561 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca 
     7621 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta 
     7681 aagggaacaa aagctggagc tgc 
//
