LOCUS       pLV-hU6-EF1a-green        8579 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     CDS             6500..7360
                     /gene=Amp(R)
     promoter        7561..8101
                     /gene=pUC ori
     promoter        7..235
                     /gene=RSV
     LTR             5105..5339
                     /gene=3'LTR
     intron          526..1690
                     /gene=Intron
     rep_origin      complement(5914..6369)
                     /gene=f1 ori
     LTR             236..416
                     /gene=5'LTR
     polyA_signal    5411..5541
                     /gene=SV40 pA
     misc_feature    5542..5755
                     /gene=SV40 ori
     promoter        2282..2677
                     /gene=human EF1a promoter
     intron          2710..3652
                     /gene=intron
     CDS             3696..4415
                     /gene=GFP
     stem_loop       1824..1875
                     /gene=shRNA
     promoter        complement(1876..2139)
                     /gene=human U6 promoter
                     
BASE COUNT     2121 a      2132 c      2216 g      2068 t        42 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 
     1861 nnnnnnnnnn nnnnnttttt ttcgtccttt ccacaagata tataaagcca agaaatcgaa 
     1921 atactttcaa gttacggtaa gcatatgata gtccatttta aaacataatt ttaaaactgc 
     1981 aaactaccca agaaattatt actttctacg tcacgtattt tgtactaata tctttgtgtt 
     2041 tacagtcaaa ttaattctaa ttatctctct aacagccttg tatcgtatat gcaaatatga 
     2101 aggaatcatg ggaaataggc cctcttcctg cccgaccttg aattcggccg gatccaactt 
     2161 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag 
     2221 caacagacat acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcga 
     2281 tgcctccccg tcaccacccc ccccaacccg ccccgaccgg agctgagagt aattcataca 
     2341 aaaggactcg cccctgcctt ggggaatccc agggaccgtc gttaaactcc cactaacgta 
     2401 gaacccagag atcgctgcgt tcccgccccc tcacccgccc gctctcgtca tcactgaggt 
     2461 ggagaagagc atgcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 
     2521 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc 
     2581 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 
     2641 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 
     2701 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc 
     2761 ccttgcgtgc cttgaattac ttccacgccc ctggctgcag tacgtgattc ttgatcccga 
     2821 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc 
     2881 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca 
     2941 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgata 
     3001 tcctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac 
     3061 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg 
     3121 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc 
     3181 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc 
     3241 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc 
     3301 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac 
     3361 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg 
     3421 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg 
     3481 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag 
     3541 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg 
     3601 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg 
     3661 aaaactaccc ctctagagat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt 
     3721 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca 
     3781 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 
     3841 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg 
     3901 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca 
     3961 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 
     4021 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 
     4081 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc 
     4141 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc 
     4201 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 
     4261 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga 
     4321 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 
     4381 ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgtcgac aatcaacctc 
     4441 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc 
     4501 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca 
     4561 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg 
     4621 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca 
     4681 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg 
     4741 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg 
     4801 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg 
     4861 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg 
     4921 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc 
     4981 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga gctcggtacc 
     5041 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 
     5101 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg 
     5161 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 
     5221 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 
     5281 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 
     5341 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga 
     5401 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 
     5461 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 
     5521 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc 
     5581 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 
     5641 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 
     5701 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg 
     5761 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg 
     5821 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt 
     5881 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg 
     5941 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 
     6001 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 
     6061 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 
     6121 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 
     6181 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 
     6241 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt 
     6301 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc 
     6361 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 
     6421 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 
     6481 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 
     6541 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 
     6601 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 
     6661 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct 
     6721 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca 
     6781 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 
     6841 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 
     6901 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 
     6961 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 
     7021 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg 
     7081 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 
     7141 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 
     7201 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 
     7261 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 
     7321 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 
     7381 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 
     7441 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 
     7501 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 
     7561 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 
     7621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 
     7681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 
     7741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 
     7801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 
     7861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 
     7921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 
     7981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 
     8041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 
     8101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 
     8161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 
     8221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 
     8281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 
     8341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 
     8401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 
     8461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 
     8521 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgc 
//
